Assessment of sperm function by using confocal laser microscopy

使用共焦激光显微镜评估精子功能

• 批准号: 05671316
• 负责人: MIYAGAWA Ikuo
• 金额: $ 0.9万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671316/
• 关键词: spermatozoom; laser microscopy; fartile potential; acrosin activity; HOST; male infertility; mid-piece

We have used semen analysis to evaluate sperm function. However, this parameter is not much correlated with fertile potential.we evaluated sperm morphometric parameters of idopathic infertile men and infertile men with valicocele by using confocal laser scanning microscopy and assested whether these parameters measured by this method were correlated with acrosin activity, HOST (hypoosmotic swelling test) , and fertile potential.We concluded as follows.1) The length of sperm mid-piece, ratio of acrosomal region of sperm head, acrosin activity, and HOST is significantly different between fertile men and infertile men.2) The length of sperm mid-piece and the ratio of acrosomal region of sperm head are much correlated with the percentage of sperm motility and acrosin activity, respectively. Inaddition, the percentage of normal morphometric spermatozoa is much correlated with the fertile potential.3) Values of sperm morphometric parameters after varicocelectomy can reliably predict sperm fertilizing potential.

我们已经使用精液分析来评估精子功能。利用激光共聚焦扫描显微镜对特发性不育男性和精索膨出不育男性的精子形态计量学参数进行了测量，并分析了这些参数与顶体酶活性、精子头部低渗肿胀试验(低渗肿胀试验)和生育潜力的相关性。结果表明：1)精子中段长度、精子头部顶体区比例、顶体酶活性和宿主在正常男性和不育男性之间存在显著差异。2)精子中段长度和精子头部顶体区比例分别与精子活动率和顶体酶活性显著相关。此外，形态正常的精子百分率与生育能力密切相关。3)精索静脉曲张手术后精子形态参数的值可以可靠地预测精子的受精能力。

项目成果

Yasuhisa Yamamoto,Koji Ono,Nikolaos Sofikitis,Ikuo Miyagawa,Yonago,Japan ; Fernado Rodriguez, Chapel Bill,NC ; Suresh Sikka,Wayne Hellstrom,New Orleans,LA (Presentation by Dr.Yamamoto).: "SPERM MORPHOMETRY ASSAY HAS HIGH PREDICTIVE VALUES FOR FERTILITY PO

山本康久、小野浩二、尼古拉斯·索菲基蒂斯、宫川郁夫、米子，日本；

NIKOLAOS SOFIKITIS.IKUO MIYAGAWA.DIMITRIOS DIMITRIADIS.PANAYIOTIS ZAVOS.SURESH SIKKA AND WAYNE HELLSTROM: "EFFECTS OF SMOKING ON TESTICULAR FUNCTION,SEMEN QUALITY AND SPERM FERTILIZING CAPACITY" The Journal of UROLOGY. Vol.154 (September). 1030-1034 (1995

NIKOLAOS SOFIKITIS.IKUO MIYAGAWA.DIMITRIOS DIMITRIADIS.PANAYIOTIS ZAVOS.SURESH SIKKA 和 WAYNE HELLSTROM：“吸烟对睾丸功能、精液质量和精子受精能力的影响”泌尿学杂志。

Nikolaos V.Sorikitis.M.D., Ph. D..<plus-minus>.* Toshiko Toda.M.D., Ph.D.* Ikuo Miyagawa.M.D., Ph.D.* Akihiro Iino.M.D., Ph.D^<****> Panaviotis M.Zavos.Ed.S., Ph.D.|| Naoki Terakawa.M.D., Ph.D.!: "Confocal scanning laser microscopy of morphometric human s

Nikolaos V.Sorikitis.M.D., Ph. D..<正负>.* Toshiko Toda.M.D., Ph.D.* Ikuo Miyakawa.M.D., Ph.D.* Akihiro Iino.M.D., Ph.D^<*

NIKOLAOS SOFIKITIS, IKUO MIYAGAWA, DIMITRIOS DIMITRIADIS, PANAYIOTIS ZAVOS, SURESH SIKKA AND WAYNE HELLSTROM: "EFFECTS OF SMOKING ON TESTICULAR FUNCTION, SEMEN QUALITY AND SPERM FERTILIZING GAPACITY" The Journal of UROLOGY. 154. 1030-1034 (1995)

NIKOLAOS SOFIKITIS、IKUO MIYAGAWA、DIMITRIOS DIMITRIADIS、PANAYIOTIS ZAVOS、SURESH SIKKA 和 WAYNE HELLSTROM：“吸烟对睾丸功能、精液质量和精子受精间隙的影响”《泌尿学杂志》。

Yasuhisa Yamamoto, Koji Ono, Nikolaos Sofikitis, Ikuo Miyagawa, Yonago, Japan ; Fernado Rodriguez. Chapel Hill, NC ; Suresh Sikka, Wayne Hellstrom, New Orleans, LA (Presentation by Dr.Yamamoto).: "SPERM MORPHOMETRY ASSAY HAS HIGH PREDICTIVE VALUES FOR FER

Yasuhisa Yamamoto、Koji Ono、Nikolaos Sofikitis、Ikuo Miyakawa，米子，日本；