Molecular Biological Analysis on Glomerular Function by Multi-Photon Confocal Laser Microscopy

多光子共焦激光显微镜对肾小球功能的分子生物学分析

• 批准号: 20300163
• 负责人: NAKAMOTO Hiroshi
• 金额: $ 12.56万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20300163/
• 关键词: 腎症; 可視化; 糖尿病; 共焦点顕微鏡; 濾過

We were able to visualize glomerular filtration in a rat. There was leakage of larger particles with the molecular weight of 70k Dal, which was found progressive even at the early stage of diabetes. The reason of this leakage was attributed to the structural changes of the slit membrane where filtration takes place. It is because alignment of podocin and CD2AP, component proteins of the slit membrane was heterogeneously damaged due to diabetes.

我们能够观察到大鼠的肾小球滤过。存在分子量为 70k Dal 的较大颗粒的渗漏，即使在糖尿病的早期阶段也发现这种渗漏是进行性的。这种泄漏的原因是由于进行过滤的狭缝膜的结构变化造成的。这是因为缝隙膜的组成蛋白podocin和CD2AP的排列因糖尿病而异质性受损。

项目成果

Visualisation of nephroprotective function of C-peptide in an early stage diabetic rat

C肽对早期糖尿病大鼠肾保护功能的可视化

• 发表时间: 2010
• 期刊: 生体医工学
• 作者: Yokoyama N;Ohsawa H;Waguri S;Machida S;Yoshikawa M;Fujimoto T;Takatani S.;Hiroshi Nakamoto
• 通讯作者: Hiroshi Nakamoto

In-vivo Visualisation of glomerular filtration by two-photon microscopy in a rat

通过双光子显微镜观察大鼠肾小球滤过的体内可视化

• 发表时间: 2009
• 期刊: IFMBE Proceedings 25
• 作者: Asoda S;Arita T;Koshitomae H;Takakuda K.;荒井謙;Zhang GX;Hiroshi Nakamoto
• 通讯作者: Hiroshi Nakamoto

糖尿病における糸球体濾過には早期から障害が生じている

糖尿病的肾小球滤过从早期就受损。

• 发表时间: 2009
• 作者: 仲本博

In-vivo Visualisation Study of Renal Microcirculation in a Rat

大鼠肾脏微循环的体内可视化研究

• 发表时间: 2010
• 作者: 仲本博;矢田豊隆;小笠原康夫;藤原宏志;Takakuda K;仲本博;Hiroshi Nakamoto
• 通讯作者: Hiroshi Nakamoto

PROGRESSION OF HYPERFILTRATION IN AN EARLY DIABETIC RAT WAS VISUALISED BY MULTIPHOTON MICROSCOPY

通过多光子显微镜观察早期糖尿病大鼠的超滤进展

• 发表时间: 2009
• 作者: Nakamoto Hiroshi;Ogasawara Yasuo;Kajiya Fumihiko
• 通讯作者: Kajiya Fumihiko