MECHANISM OF ALCOHOLIC TESTICULAR DAMAGE

酒精损伤睾丸的机制

• 批准号: 05671340
• 负责人: IKEMOTO Isao
• 金额: $ 1.41万
• 依托单位: JIKEI UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671340/
• 关键词: ALCOHOL; TESTICULAR DAMAGE; SPERMTOGENESIS; TESTOSTERONE; ALCOHOL DEHYDROGENASE; 精巣; ラット; 栄養素

To observe the mechanism of alcoholic testicular damage, in a previous experiment we used weanling male SD rats aged 45 days, weighing about 200g, and fed a liquid diet (Lieber's) containing 5% ethanol. The latter accounted for 36% of total caloric intake for 7 weeks, but did not result in testicular atrophy. In a later experiment, we used a liquid diet in which ethanol accounted for 46% of the total calorie count. It provided a high-fat, low-protein content which simulated the nutritional background of patients with alcholic liver diseases. This diet resulted in testicular atrophy. Histological and biochemical changes accompanying this experimental testicular atrophy included the following :1) The testes of alcohol-fed animals contained smaller seminiferous tubules with reduced numbers of total cells, but no degeneration was seen in the spermatids.2) In the peritubular wall of the seminiferous tubules, we observed curvature, irregularities, infolding of the basement membrane, and lame … More llation of the lamina densa, as well as hyperplasia of collagen fibers in the tunica propria.3) In the cytoplasm of the Sertoli cells, deposits of gigantic fat droplets and stratification of the mitochondria were observed. The permeability of the Sertoli cell tight junction was confirmed using the Lanthanum method.4) Testosterone levels in both the serum and testes declined.5) Lactate dehydrogenase-X (LDH-X) activity in the tastes declined.6) Low Km alcohol dehydrogenase (ADH) activity localized in the testicular interstitial tissue was increased.These results indicate that the composition of three major nutritional elements as well as alcohol concentration are important in the mechanism of alcoholic testicular damage, and this damage affects both the testicular interstitial cell and the seminiferous tubules, particularly the Sertoli cells and peritubular wall of the latter. In addition, the findings suggest that ADH is involved in alcohol metabolism in the interstitial cells of the testes. Less

为了观察酒精性睾丸损伤的机制，在之前的实验中，我们使用45日龄断奶雄性SD大鼠，体重约200g，饲喂含5%乙醇的流质饲料（Lieber's）。后者在7周内占总热量摄入的36%，但并未导致睾丸萎缩。在后来的实验中，我们使用了流质饮食，其中乙醇占总热量的 46%。它提供高脂肪、低蛋白质含量，模拟酒精性肝病患者的营养背景。这种饮食导致睾丸萎缩。伴随该实验性睾丸萎缩的组织学和生化变化包括以下内容：1）酒精喂养的动物的睾丸含有较小的生精小管，细胞总数减少，但精子细胞中未见变性。2）在生精小管的管周壁中，我们观察到弯曲、不规则、基底膜内皱和跛行……更多 致密层变薄，固有膜胶原纤维增生。3)支持细胞胞浆内可见巨大脂肪滴沉积和线粒体分层。使用镧法证实了支持细胞紧密连接的通透性。4）血清和睾丸中的睾酮水平下降。5）味觉中的乳酸脱氢酶-X（LDH-X）活性下降。6）睾丸间质组织中的低Km乙醇脱氢酶（ADH）活性增加。这些结果表明，三种主要营养成分的组成 元素和酒精浓度在酒精性睾丸损伤的机制中很重要，这种损伤同时影响睾丸间质细胞和生精小管，特别是支持细胞和后者的管周壁。此外，研究结果表明ADH参与睾丸间质细胞的酒精代谢。较少的

项目成果

SHIRAI T.AND IKEMOTO I.: "MECHANISM OF TESTICULAR DAMAGE" JPN J UROL.83-3. 305-314 (1992)

Shirai T. 和 ikemoto I.：“睾丸损伤机制”JPN J UROL.83-3。

白井尚、池本庸: "アルコール性精巣障害の発現機序" 日本泌尿器科学会雑誌. 83. 305-314 (1992)

Hisashi Shirai，Tsune Ikemoto：“酒精性睾丸功能障碍的表达机制”日本泌尿外科协会杂志 83. 305-314 (1992)。

西田篤、白井尚、池本庸: "男子不妊症患者における精路・副性器感染症の影響" 東京慈恵会医科大学雑誌. 108. 821-827 (1993)

Atsushi Nishida、Hisashi Shirai 和 Tsune Ikemoto：“男性不育患者精道和副生殖道感染的影响”东京慈惠大学医学院学报 108. 821-827 (1993)。

NICHIDA A., SHIRAI T.AND IKEMOTO I.: "EFFECT OF MALE ACCESSORY GLAND INFECTION ON MALE INFERTILITY PATIENT"

NICHIDA A.、SHIRAI T. 和 IKEMOTO I.：“男性附件腺感染对男性不育症患者的影响”

西田 篤: "男性不妊症患者における精路.副性器感染症の影響" 東京慈恵会医科大学雑誌. 108. 821-827 (1993)

Atsushi Nishida：“生殖道感染对男性不育患者的影响”东京慈惠大学医学院学报 108. 821-827 (1993)