MECHANISM OF ALCOHOLIC TESTICULAR DAMAGE

为了观察酒精性睾丸损伤的机制，在先前的实验中，我们使用了45日龄的断奶雄性SD大鼠，体重约200 g，并喂食含有5%乙醇的液体饲料（Lieber's）。后者占7周总热量摄入的36%，但未导致睾丸萎缩。在后来的实验中，我们使用了一种液体饮食，其中乙醇占总热量的46%。它提供了高脂肪、低蛋白含量，模拟了酒精性肝病患者的营养背景。这种饮食导致睾丸萎缩。实验性睾丸萎缩的组织学和生化变化包括：1）酒精喂养动物的睾丸含有较小的曲细精管，细胞总数减少，但精子细胞未观察到变性; 2）在曲细精管的管周壁，我们观察到弯曲、不规则、基底膜内折和跛脚。 ...更多信息 3）Sertoli细胞胞质内可见巨大脂肪滴沉积和线粒体分层。用镧法证实了睾丸支持细胞紧密连接的通透性。4）血清和睾丸中睾酮水平下降。5）味觉中乳酸脱氢酶-X（LDH-X）活性下降。6）低Km乙醇脱氢酶（ADH）。这些结果表明，三种主要营养元素的组成以及酒精浓度，在酒精性睾丸损伤的机制中起重要作用，这种损伤影响睾丸间质细胞和曲细精管，特别是后者的支持细胞和管周壁。此外，研究结果表明ADH参与了睾丸间质细胞的酒精代谢。少

To observe the mechanism of alcoholic testicular damage, in a previous experiment we used weanling male SD rats aged 45 days, weighing about 200g, and fed a liquid diet (Lieber's) containing 5% ethanol. The latter accounted for 36% of total caloric intake for 7 weeks, but did not result in testicular atrophy. In a later experiment, we used a liquid diet in which ethanol accounted for 46% of the total calorie count. It provided a high-fat, low-protein content which simulated the nutritional background of patients with alcholic liver diseases. This diet resulted in testicular atrophy. Histological and biochemical changes accompanying this experimental testicular atrophy included the following :1) The testes of alcohol-fed animals contained smaller seminiferous tubules with reduced numbers of total cells, but no degeneration was seen in the spermatids.2) In the peritubular wall of the seminiferous tubules, we observed curvature, irregularities, infolding of the basement membrane, and lame … More llation of the lamina densa, as well as hyperplasia of collagen fibers in the tunica propria.3) In the cytoplasm of the Sertoli cells, deposits of gigantic fat droplets and stratification of the mitochondria were observed. The permeability of the Sertoli cell tight junction was confirmed using the Lanthanum method.4) Testosterone levels in both the serum and testes declined.5) Lactate dehydrogenase-X (LDH-X) activity in the tastes declined.6) Low Km alcohol dehydrogenase (ADH) activity localized in the testicular interstitial tissue was increased.These results indicate that the composition of three major nutritional elements as well as alcohol concentration are important in the mechanism of alcoholic testicular damage, and this damage affects both the testicular interstitial cell and the seminiferous tubules, particularly the Sertoli cells and peritubular wall of the latter. In addition, the findings suggest that ADH is involved in alcohol metabolism in the interstitial cells of the testes. Less