A flow cytometric DNA analysis ofthe reversible testicular damage in the rat and the effect of mecobalamin on the recovery of spermatogenesis

本研究评价了应用DNA流式细胞术检测和定性盐酸阿霉素（ADR）诱导的精子发生可逆性损伤。雄性Sprague-Dawley（S-D）大鼠皮下注射ADR，剂量为0.1、0.25或0.5 mg/kg，每周3次，持续3、5或7周。对照组按照相同的三种方案用生理盐水处理。通过对大鼠体重、睾丸重量的变化及DNA流式细胞术分析，认为ADR 0.25mg/kg连续5周是一种较为理想的可逆性睾丸损伤大鼠模型，并在此基础上，观察甲钴胺（MBL）对精子发生恢复过程的影响。雄性S-D大鼠皮下注射ADR 0.25mg/kg，每周3次，共5周，然后分别口服阿霉素0.01mg/kg、0.1mg/kg和1 mg/kg，每周6次。治疗后5周、10周和15周，提取睾丸并进行流式细胞术DNA分析。0.1mg/kg MBL组15周时的恢复率显著高于对照组（仅ADR），提示该动物模型可用于快速、定量评价化学药物对精子发生的影响，MBL可促进实验性精子发生损伤的恢复过程。

This study evaluated the use of DNA flow cytometric analysis to detect and quality reversible damage in spermatogenesis induced by doxorubicin hydrochloride(ADR). Male Sprague-Dawley(S-D) rats were treated subcutaneously with ADR at a dose of 0.1, 0.25 or 0.5 mg/kg, 3 times a week for either 3, 5 or 7 weeks. Controls were treated with either saline following the same three regimens. Evaluation of changes in body and testicular weights, as well as DNA flow cytometric analysis, revealed that rats treated with ADR at 0.25 mg/kg for 5 weeks were the most suitable as a reversible testicular damage rat model.Standing on the above established animal model, the effect mecobalamin (MBL) on the recovery process of spermatogenesis was investigated. Male S-D rats were subcutaneously injected with ADR 0.25 mg/kg, 3 times week, for 5 weeks, after which rats were orally adiministered with MBL 0.01 mg/kg, 0.1 mg/kg and 1mg/kg, 6 times a week. Five, ten and fifteen weeks after the treatment, testicles extracted and flow cytometyric DNA analysis was performed. In the group treated with 0.1 mg/kg of MBL, significantly greater recovery was revealed compared with the control group(only ADR) at 15 week.These results suggest that this animal model is useful for rapid and quantitative assessment of the effect the chemical agenct on spermatogenesis and that MBL promoted the recovery process of experimentally damaged spermatogegesis.