A flow cytometric DNA analysis ofthe reversible testicular damage in the rat and the effect of mecobalamin on the recovery of spermatogenesis

本研究评估了DNA流式细胞分析在检测和评价盐酸阿霉素（ADR）诱导的精子发生的可逆性损伤中的应用。雄性Sprague-Dawley（S-D）大鼠皮下注射ADR，剂量分别为0.1、0.25或0.5 mg/kg，每周3次，持续3、5或7周。对照组按照同样的三种治疗方案使用生理盐水。机体和睾丸重量变化及DNA流式细胞术分析显示，0.25 mg/kg剂量的ADR治疗5周后最适合作为可逆性睾丸损伤大鼠模型。在上述建立的动物模型上，研究甲钴胺（MBL）对精子发生恢复过程的影响。雄性S-D大鼠皮下注射ADR 0.25 mg/kg，每周3次，连续5周，然后分别口服MBL 0.01 mg/kg、0.1 mg/kg和1mg/kg，每周6次。治疗后5周、10周和15周，提取睾丸并进行流式细胞术DNA分析。以0.1 mg/kg MBL治疗组，与对照组相比，15周恢复明显更大（只有不良反应）。这些结果表明，该动物模型有助于快速定量评价化学制剂对精子发生的影响，MBL促进了实验损伤精子发生的恢复过程。

This study evaluated the use of DNA flow cytometric analysis to detect and quality reversible damage in spermatogenesis induced by doxorubicin hydrochloride(ADR). Male Sprague-Dawley(S-D) rats were treated subcutaneously with ADR at a dose of 0.1, 0.25 or 0.5 mg/kg, 3 times a week for either 3, 5 or 7 weeks. Controls were treated with either saline following the same three regimens. Evaluation of changes in body and testicular weights, as well as DNA flow cytometric analysis, revealed that rats treated with ADR at 0.25 mg/kg for 5 weeks were the most suitable as a reversible testicular damage rat model.Standing on the above established animal model, the effect mecobalamin (MBL) on the recovery process of spermatogenesis was investigated. Male S-D rats were subcutaneously injected with ADR 0.25 mg/kg, 3 times week, for 5 weeks, after which rats were orally adiministered with MBL 0.01 mg/kg, 0.1 mg/kg and 1mg/kg, 6 times a week. Five, ten and fifteen weeks after the treatment, testicles extracted and flow cytometyric DNA analysis was performed. In the group treated with 0.1 mg/kg of MBL, significantly greater recovery was revealed compared with the control group(only ADR) at 15 week.These results suggest that this animal model is useful for rapid and quantitative assessment of the effect the chemical agenct on spermatogenesis and that MBL promoted the recovery process of experimentally damaged spermatogegesis.