STUDY OF THE ROLES OF PTH RELATED PEPTIDE (PTHrp) IN REPRODUCTION AND GYNECOLOGIC TUMOR

(1)PTHrP基因在正常女性生殖器官中的表达：(1)RT-PCR证实PTHrP基因在子宫内膜、蜕膜、绒毛、输卵管、卵巢、子宫肌以及胎膜和胎肝、肠、皮肤中均有表达。(2)免疫组织化学分析显示，PTHrP在子宫内膜细胞、蜕膜组织中的间质细胞、绒毛膜细胞、蜕膜间质细胞、绒毛组织中的合体中均呈阳性表达。(3)在血清PTHrP浓度较高的哺乳期产妇乳汁中，PTHrP呈高水平表达。提示哺乳期产妇发生了乳中PTHrP向血流的转移。(4)妊娠中期羊水中PTHrP浓度最高。(5)卵泡液中PTHrP含量较高。(2)PTHrP基因在卵巢良性肿瘤组织中的表达及卵巢肿瘤患者PTHrP的临床意义。(2)PTHrP基因在卵巢良、恶性肿瘤组织中的表达及临床意义。(B)用放射免疫法测定34例卵巢良性肿瘤患者血清中PTHrP浓度。卵巢恶性肿瘤27例，其中良性肿瘤5例，恶性肿瘤10例。8%的良性肿瘤患者和35%的恶性肿瘤患者血清甲状旁腺素rP水平升高。33%的恶性肿瘤腹水和囊液中PTHrP浓度很高(>500pmol/L)。这些结果表明，PTHrP测定在某些卵巢肿瘤中是有用的肿瘤标志物。

(1) PTHrP gene expression and presence of PTHrp molecule in normal female reproductive organs.(a) PTHrP gene expression was confirmed by means of RT-PCR in endometrium, decidua, villi, tube, ovary, and uterine muscle as well as fetal membrane and fetal liver, intestine and skin.(b) Immunohistochemical analysis showed that PTHrP was positively stained in endometrial cells in endometrium, endometrial cells, stromal cells in decidual tissue, chorionic cells, decidual stromal cells in fetal membrane, syntijtium in chorionic villi.(c) High concentration of PTHrP was present in milk of lactating puerperal women, in whom serum PTHrP concentration was high, suggesting the transfer of milk PTHrP to the blood stream of lactating puerperal women occured.(d) PTHrP concentration in amniotic fluid was the highest in second trimester during pregnancy. Amniotic fluid PTHrP concentration in hydroamnios and pregnancies with anomalous fetus was not different from normal controls expect for thanatophoric dwrafism in which PTHrP concentration was lower.(e) Follicular fluid contained high concentration of PTHrP.PTHrp levels seemed not tobe affected by estrogen or progesterone levels in follicular fluid.(2) PTHrP gene expression in tissues of ovarian benign and malignant tumors and clinical signific ance of PTHrP in patients with ovarian tumors.(a) PTHrP gene expression was seen in tissue of both benign and malignant tumors.(b) PTHrP concentration was determined by means of RIA in sera of patients with benign ovarian tumors (n=34), malignant ovarian tumors (n=27) and ascites and cystic fluid (from benign tumors, n=5, malignant tumors, n=10). High serum levels of PTHrP were seen in 8 % of cases with benign tumors and in 35 % of cases with malignant tumors. Concentration of PTHrP in asctic and cystic fluid was very high (more than 500 pmol/L) in 33 % of malignant cases. These results suggest that PTHrP determination is useful a tumor marker in some cases of ovarian tumors.