Screening of fluorescently detectable RNA using evolutionary molecular engineering

为了获得细胞中自发荧光的RNA，我们在E.Coli中表达了随机RNA文库，并对其荧光特性进行了分析。我们构建了与FMN(黄素单核苷酸)结合的RNA文库，并在大肠杆菌中表达了该文库。我们分析了10000000个E.Coli克隆的荧光特性，但尚未获得预期的荧光核糖核酸。

To obtain the spontaneously fluorescent RNA in cells, we have expressed the randomized RNA library in E. Coli and analyzed the fluorescent characteristics in E. Coli. We have constructed RNA library binding to FMN (flavin mono nucleotide), which emits fluorescence and ubiquitously dispersed all over cells, and expressed the cDNA library in E. Coli. We analyzed the fluorescent characteristics of 10000000 E.Coli clones, however, we have not obtained the expected fluorescent RNA yet.