Screening of fluorescently detectable RNA using evolutionary molecular engineering

利用进化分子工程筛选可荧光检测的RNA

• 批准号: 23655165
• 负责人: KATO Yoshio
• 金额: $ 1.41万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23655165/
• 关键词: 核酸; 蛍光性ＲＮＡ; 進化分子工学; RNA; 大腸菌; 蛍光; 分子イメージング

To obtain the spontaneously fluorescent RNA in cells, we have expressed the randomized RNA library in E. Coli and analyzed the fluorescent characteristics in E. Coli. We have constructed RNA library binding to FMN (flavin mono nucleotide), which emits fluorescence and ubiquitously dispersed all over cells, and expressed the cDNA library in E. Coli. We analyzed the fluorescent characteristics of 10000000 E.Coli clones, however, we have not obtained the expected fluorescent RNA yet.

为了获得细胞内自发荧光的RNA，我们在E.大肠杆菌中，并分析了大肠杆菌的荧光特性。杆菌我们构建了与荧光素FMN（flavinmononucleotide）结合的RNA文库，并在E.杆菌我们分析了1000万个大肠杆菌克隆的荧光特性，但是，我们还没有得到预期的荧光RNA。

项目成果

新規蛍光標識核酸

新型荧光标记核酸

• 发表时间: 2006