Catecholamine synthetic capacity and dopamine receptors in fetal rat lung

胎鼠肺中儿茶酚胺合成能力和多巴胺受体

• 批准号: 05671371
• 负责人: KUDO Takafumi
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671371/
• 关键词: Fetal lung; Catecholamine; Dopamine; Dopa decarboxylase; Growth-retarded fetus; Chronic fetal hypoxia; Fetal stress; 胎仔肺機能; 胎児慢性低酸素症; ドパミン受容体

Catecholamine (CA) synthetic capacity in fetal rat lung was cxamined on Day 20 of gestation. Three common CAs were present in the fetal rat lung. But their proportions differed from those in the adrenal gland, brain, blood, and amniotic fluid. To confirm the capacity of the fetal rat lung to synthesize CA,L-dopa was injected into pregnant rats, and CAs in the lungs of the fetuses of these rats were measured. The concentrations of all three CAs were increased significantly as compared with those in the lungs of fetuses from control rats. Immunohistochemical technique served to localize the CA synthetic enzyme, aromatic-L-amino acid decarboxylase, in the lung. The concentration of CAs in the lungs of growth-retarded rat fetuses prepared by the Wigglesworth method was higher than in the lungs of normal fetuses. Thus, it appeared that CAs exist in the fetal rat lung, and that fetal lung can synthesize all CAs. Findings in growth-retarded fetuses suggest that CA content in the fetal lung may be altered under conditions of chronic hypoxia.

于孕20天测定胎鼠肺组织中儿茶酚胺（CA）的合成能力。三种常见的CA存在于胎鼠肺中。但它们的比例与肾上腺、大脑、血液和羊水中的比例不同。为了确认胎鼠肺合成CA的能力，将L-多巴注射到妊娠大鼠中，并测量这些大鼠的胎鼠肺中的CA。与对照组大鼠胎鼠肺组织相比，三种CA的浓度均显著升高。免疫组化技术定位CA合成酶，芳香族-L-氨基酸脱羧酶，在肺。Wigglesworth法制备的生长迟缓胎鼠肺中CA的浓度高于正常胎鼠肺中CA的浓度。由此可见，CA存在于胎鼠肺中，且胎肺可合成所有CA。生长迟缓胎儿的研究结果表明，在胎儿肺CA含量可能会改变慢性缺氧条件下。

项目成果

Tada K,Kishimoto Y,Arai F,Kondo Y,Yanagawa T and Kudo T: "Fetal maturation and catecholamine metabolism." Perinatal Care. 14. 349-356 (1995)

Tada K、Kishimoto Y、Arai F、Kondo Y、Yanakawa T 和 Kudo T：“胎儿成熟和儿茶酚胺代谢。”

中谷 義明他: "Catecholamine synthetic capacity in fetal rat lung;Changes with growth-retardation" Asia-Oceania Journal of Obstetrics and Gynaecology. 20 (発表予定). (1994)

Yoshiaki Nakatani 等人：“胎鼠肺中的儿茶酚胺合成能力；生长迟缓的变化”亚洲-大洋洲妇产科杂志 20（待出版）。

Kishimoto Y,Tada K,Yanagawa T,Kondo Y,Arai F,Inoue K,Hiraoka K,Kawada A,Mitsuya H and Yashiro Y: "Changes in catecholamine metabolism during perinatal period." ACTA NEONAT.JPN.31. 647-652 (1995)

Kishimoto Y、Tada K、Yanakawa T、Kondo Y、Arai F、Inoue K、Hiraoka K、Kawada A、Mitsuya H 和 Yashiro Y：“围产期儿茶酚胺代谢的变化”。

多田 克彦: "胎児成熟とカテコラミン代謝" ペリネイタルケア. 14. 349-359 (1995)

Katsuhiko Tada：“胎儿成熟和儿茶酚胺代谢”围产期护理 14. 349-359 (1995)。

岸本 廉夫: "IUGR" 臨床婦人科産科. 48. 1008-1009 (1994)

岸本直树：“IUGR”临床妇产科 48. 1008-1009 (1994)。