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Characteristics of experimentally induced foreign-body type giant cells

实验诱导异物型巨细胞的特征

基本信息

批准号：
05671529
负责人：
EDA Shigeo
金额：
$ 1.28万
依托单位：
Matsumoto Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

The purpose of this investigation is to clarify the occurrence and enzyme-histochemical and morphological characteristics of multinucleated giant cells induced by some foreign bodies (HAP,TCP,BP,and CHOL.These materials were subcutaneously embedded in rats. To evaluate the enzyme-histochemical characteristics of the giant cells, the positive area ratio of cells reacting positively to ACP,TRAP,CCAP,and CCTP were measured by means of image analysis system.On the bases these observations, the following results are obtained. 1) Giant cells form as the result of the fusion of many macrophage-like mononuclear cells. 2) Giant cells form by mitosis without cytoplasmic division. Furthermore, in terms of enzyme-histochemical image analysis, positive reactions to ACP,TRAP,CCAP,and CCTP are though to have occurred 1 week after giant cell formation. Positive area ratio were in descending order HAP,TCP,BP,and CHOL.There were statistically significant difference (P<0.01 ; Scheffe test) among these groups. The ratio peaks were as follows : HAP-2 weeks, TCP-3 weeks, BP-4 weeks, and CHOL-1 week specimens.Although a tendency to react positively to TRAP was observed in phagocytization of such calcium phosphate ceramics, it seemed impossible to identify elicited giant cells as osteoclasts sullenly on the basis of their positive reaction to TRAP.Even though the giant cells demonstrate osteoclastlike features of enzyme-histochemical characteristics, they elicited in this study still may belong to the foreign body type giant cells.

本研究的目的是阐明几种异物（HAP、TCP、BP和CHOL）诱发的大鼠皮下多核巨细胞的发生、酶组织化学和形态学特征。为了评价巨细胞的酶组织化学特征，应用图象分析系统测定了巨细胞对ACP、TRAP、CCAP和CCTP的阳性反应面积比，结果如下：1)巨细胞是许多巨噬细胞样单核细胞融合的结果。2)巨细胞通过有丝分裂而不进行胞质分裂形成。此外，在酶组织化学图像分析方面，ACP、TRAP、CCAP和CCTP的阳性反应被认为在巨细胞形成后1周发生。阳性面积率依次为HAP、TCP、BP、CHOL，各组间差异有统计学意义（P<0.01 ; Scheffe检验）。比值峰如下：HAP-2周、TCP-3周、BP-4周和CHOL-1周的样品。尽管在这种磷酸钙陶瓷的吞噬中观察到对TRAP阳性反应的趋势，基于对TRAP的阳性反应，似乎不可能将诱导的巨细胞鉴定为破骨细胞。组织化学特征，本研究所引出的它们仍可能属于异物型巨细胞。