Screening of arbuscular mycorrhizal fungi with high acid phosphatase secretion and it utilization

为阐明丛枝菌根真菌对土壤有机磷的利用，对丛枝菌根真菌根外菌丝酸性磷酸酶（ACP）活性进行了研究。用AM真菌Rhizophagus clarus接种或未接种大葱幼苗，并在用30 μm孔径的尼龙网分成根和菌丝室的盆中生长。菌丝室内土壤溶液中ACP活性在接种A. fistulosum比未接种的植物。接种植株体内ACP活性在低磷条件下高于高磷条件。这些结果表明，AM真菌R.在缺磷条件下，该酶的活性被诱导。

Activity of acid phosphatase (ACP) from extraradical hyphae of arbuscular mycorrhizal (AM) fungi was studied in order to clarify utilization of soil organic phosphorus by AM fungi. Seedlings of Allium fistulosum were inoculated or uninoculated with AM fungi Rhizophagus clarus and were grown in pots that were separated into root and hyphal compartments with nylon net of 30 µm pore size. ACP activity of soil solution in hyphal compartment was higher in inoculated A. fistulosum than uninoculated plant. ACP activity in inoculated plant was higher at low soil P condition than that at high soil P condition. These results suggest that extraradical hyphae of AM fungi R. clarus exude ACP and its activity is induced under P deficient condition.