Effect of chemotherapeutic agent on leukocyte function

观察了氨苄青霉素（ABPC）、头孢氨苄（CEX）、头孢替安（CTM）、阿米卡星（AMK）、克林霉素（CLDM）、四环素（TC）和博莱霉素（BLM）等7种抗生素对大鼠巨噬细胞（MPHI）对酵母多糖激活血清（ZAS）趋化性的影响。用48孔微趋化室膜过滤法测定大鼠腹腔MPHI对ZAS的趋化性，然后将大鼠MPHI与每种抗生素孵育。所有抗生素剂量依赖性地抑制大鼠MPHI趋化性。在1 μ g/ml时，除ABPC和CEX外，所有抗生素均显著抑制大鼠对ZAS的MPHI趋化性（CLDM：p<0.05，AMK：p<0.01，CTM、TC和BLM：p<0.001）。在10 μ g/ml浓度下，抗生素显著抑制大鼠对ZAS的MPHI趋化作用（ABPC和CLDM：p<0.01，其他抗生素：p<0.001）。在100 μ g/ml时，抗生素显著抑制大鼠MPHI趋化性（p<0.001）。除ABPC外，所有抗生素在10- 1000 μ g/ml或更高剂量时，吞噬率和吞噬指数均呈剂量依赖性下降，CTM、CEX、TC、AMK和CLDM在100- 1000 μ g/ml或更高剂量时，吞噬率显著下降（p<0.001）。CTM、CEX和CLDM组吞噬指数均显著降低（p<0.01：1000 μ g/ml）。TC（p<0.001：1000 μ g/ml）和AMK（p<0.001：1000 μ g/ml和p<0.001：1000 μ g/ml）。BLM可显著降低巨噬细胞的吞噬率（p<0.001：10- 1000 μ g/ml）和吞噬指数（p<0.001：10 μ g/ml，p<0.001：100- 1000 μ g/ml）。ABPC的吞噬率和吞噬指数呈剂量依赖性增加，但与对照组无显著差异。这些发现表明，通过感染或免疫宿主防御的MPHI的积累是通过抑制MPHI趋化性和抗生素的吞噬作用来维持的，并且这种功能促进了感染的愈合。

The effect of seven kinds of antibiotics, ampicillin (ABPC), cephalexin (CEX), cefotiam (CTM), amikacin (AMK), clindamycin (CLDM), tetracyline (TC) and bleomycin (BLM )on the rat macrophage (MPHI) chemotaxis to zymosan activated serum (ZAS) was examined. The chemotaxis of rat peritoneal MPHI to ZAS was measured by the membrane filter method using 48-well microchemotaxis chambers and then the rat MPHIs were incubated with each antibiotic. AII antibiotics dose-dependently suppressed the rat MPHI chemotaxis. At 1 mug/ml all the antibiotics, except ABPC and CEX,inhibited rat MPHI chemotaxis to ZAS significantly (CLDM : p<0.05, AMK : p<0.01, CTM,TC and BLM : p<0.001). At 10mug/ml, the antibiotics inhibited rat MPHI chemotaxis to ZAS significantly (ABPC and CLDM : p<0.01, other antibiotics : p<0.001). At 100mug/ml, the antibiotics markedly suppressed rat MPHI chemotaxis (p<0.001). All antibiotics except ABPC showed dose-dependent decrease of both phagocytosis ratio and index at doses of 10-1000mug/ml or more.CTM,CEX,TC,AMK and CLDM showed significant decrease of phagocytosis ratio (p<0.001) at the dose of 100-1000mug/ml or more. Significant decrease of phagocytosis index were shown in CTM,CEX and CLDM (p<0.01 : 1000mug/ml). TC (p<0.001 : 1000mug/ml) and AMK (p<0.01 : 100mug/ml and p<0.001 : 1000mug/ml). BLM showed significant decrease of phagocytosis ratio (p<0.001 : 10-1000mug/ml) and phagocytosis index (p<0.01 : 10mug/ml, p<0.001 : 100-1000mug/ml). ABPC slightly showed dose-dependent increase of both phagocytosis ratio and index, but was not significantly different from those of controls. These findings suggest that the accumulation of MPHIs by infection or immunological host defense is maintained by the inhibition of MPHI chemotaxis and phagocytosis of antibiotics and that this function promotes the healing of infecion.