Effect of insulin on naturally occurring gingivitis rats with diabetes

糖尿病患者mellitus causes aggravation of periodontal diseasesand the relationship between diabetes mellitus and periodontal disease已被examined我们used naturally occurring gingivitis rat (ODUS/Odu) to induce diabetes mellitus with induce diabetesan injection of streptozotocin (STZ：65mg/kg, i.v.). 4 weeks after STZ injection，we administered insulin daily to a second group (STZ-Insulin：STZ- in). Eight weeks after STZ injectionsterilized liquid paraffin was injected peritoneally. 4 days later，peritoneal macrophages collected from the 3 groups (STZ, STZ- in, Control：The chemotaxis of macrophages membrane filter method using a 48-wellmicrochemotaxis chamber. Zymosan activated serum used as a stimulant of macrophage chemotaxis.Moreover, we measured body weight, blood glucose, plaque index，pocket probing depths （at the center of each mandibular incisor）triglyceride and hemoglobin A D21C D2. In the STZ-In group，the body weight and blood glucose levels had learly recovered. Pocket probing depths in STZ wasMacrophage chemotaxis on STZ was significantly decreased compared to Macrophage chemotaxis on STZ was significantly decreasedcont,但没有显著的差异STZ和STZ- in . The findings suggested that insulinhas only a slight recovery effect on pocket probing depth or chemotaxis。

Diabetes mellitus causes aggravation of periodontal diseases, and the relationship between diabetes mellitus and periodontal disease has been examined extensively. We used naturally occurring gingivitis rat (ODUS/Odu) to induce diabetes mellitus with an injection of streptozotocin (STZ : 65mg/kg, i.v.). Four weeks after STZ injection, we administered insulin daily to a second group (STZ-Insulin : STZ-In). Eight weeks after STZ injection, sterilized liquid paraffin was injected peritoneally. Four days later, the peritoneal macrophages were collected from the 3 groups (STZ, STZ-In, Control : Cont). The chemotaxis of macrophages was measured by the membrane filter method using a 48-well microchemotaxis chamber. Zymosan activated serum was used as a stimulant of macrophage chemotaxis. Moreover, we measured body weight, blood glucose, plaque index, pocket probing depths (at the center of each mandibular incisor), triglyceride and hemoglobin AィイD21CィエD2. In the STZ-In group, the body weight and blood glucose levels had clearly recovered. Pocket probing depths in STZ was significantly deeper than Cont. Macrophage chemotaxis on STZ was significantly decreased compared to Cont, but there was no significant difference between STZ and STZ-In. The findings suggested that insulin has only a slight recovery effect on pocket probing depth or chemotaxis.