Experimental study on jaw reconstruction using bovine bone morphogenetic protein augmented bone substitute and allogeneic preserved bone

牛骨形态发生蛋白增强骨替代物与同种异体保存骨重建颌骨的实验研究

• 批准号: 05671681
• 负责人: HORIUTI Katsuhiro
• 金额: $ 1.34万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671681/
• 关键词: BMP; Mandibular reconstruction; Allogeneic bone; Atelocollagen; Osteogenesis; 下顎骨再建; 骨誘導; 同種骨移植; 保存骨

A sintered bone had the lowest antigenicity among a variety of allogeneic preserved bones, followed by autoclaved, demineralized, lyophilized, frozen, partially demineralized, and defatted bones.When combined with rat allogeneic preserved bone and bovine bone morphogenefic protein (BMP) augmented bone substitute, demineralized and lyophilized bones were the most excellent in osteoinductivity and biomechanical strength, respectively.In a defect after marginal mandibulectomy, the implants described above with bone marrow induced more bone formation than they alone. Combination with bone marrow resulted in no significant differences in osteogenesis between demineralized and lyophilized bones. In biomechanical strength, however, the lyophilized bone had superior results regardless combination with bone marrow. There were no significants differences in responses of surrounding soft-tissues on implants among experimental groups.In implantation into a mandibular segmental defect, the unstability of implant materials prevents the experiment from progressing. Moreover, the result from the rat experiment seems difficult to suggest a possibility of clinical applications. Therefore, it is necessary to perform the same experiments using a rabbit, dog or monkey, and we are now in progress.

在各种异体保存骨中，烧结骨的抗原性最低，其次是高压灭菌、脱矿、冻干、冷冻、部分脱矿和脱脂骨。当与大鼠同种异体保存骨和牛骨形态发生蛋白（BMP）增强骨替代物联合使用时，去矿化骨和冻干骨的成骨性和生物力学强度分别最好。在下颌骨边缘切除术后的缺损中，上述骨髓植入物比单独植入物诱导更多的骨形成。与骨髓结合导致脱矿和冻干骨的成骨无显著差异。然而，在生物力学强度方面，无论与骨髓结合，冻干骨都有更好的结果。各组间种植体周围软组织的反应无明显差异。在下颌骨缺损种植中，种植体材料的不稳定性阻碍了实验的进展。此外，大鼠实验的结果似乎很难提出临床应用的可能性。因此，有必要用兔子、狗或猴子进行同样的实验，我们现在正在进行中。