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Cell Biological Study on the Response Against Mechanical Stress on Periodontal Ligament Fibroblasts Derived from Human Deciduous Teeth.

人乳牙牙周膜成纤维细胞对机械应力反应的细胞生物学研究。

基本信息

批准号：
05671726
负责人：
KIMOTO Shigenari
金额：
$ 1.34万
依托单位：
Kanagawa Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Periodontal ligament fibroblasts derived from human deciduous (HPLF-Y) and permanent (HPLF) teeth were established by means of explant method reported by Kawase and Saito. The effects of mechanical stress on cell growth and differentiation were studied on HPLF-Y and HPLF in culture. Those cells were loaded with intermittent mechanical strain at 5 or 10% elongation for 10 second with the resting for 10 or 50 second using the flexible bottomed culture plates and vacuum system in the presence or absence of retinoic acid (RA), 1,25 dihydroxy vitamin D_3 (D_3) or both of them. After the stretching for 24 hours, we measured alkaline phosphatase (ALPase) activity and DNA quantity of both cells. DNA synthesis of HPLF-Y and HPLF were suppressed by treatment with 0.1 mM RA for 8 days. The growth of both cells was more suppressed by RA treatment in the latter half of culture period than by D_3 or by RA and D_3 together. Alkaline phosphatase (ALPase) activity of HPLF-Y was increased by treatment w … More ith 0.1 mM RA for 8 days, however no significant change was observed on HPLF after treatment with RA at the same concentration. After the loading of intermittent stretching (5%, 3 cycle/min., 24 hr) on the day 5 in culture, cell growth of HPlF was stimulated, whereas that of HPLF-Y was suppressed. On the other hand, ALPase synthesis was stimulated on both HPLF-Y and HPLF.The mechanical stress (5%) caused significant increase of ALPase on HPLF-Y treated with D_3 or with RA and D_3 together at confluent phase. ALPase activity was increased by stretching at growth phase of HPLF treated with RA or D_3 separately or with RA and D_3 together. These data demonstrate that there are obvious differences between HPLF-Y and HPLF in terms of the response to RA or mechanical stress. The present study suggests that HPLF-Y and HPLF have different functions in the remodeling of periodontal tissue during development and that D_3 and RA are able to modify of the response to the mechanical strain on the cell growth and differentiation of HPLF and HPLF-Y. Less

采用Kawase和Saito报道的植块法建立了人乳牙和恒牙牙周膜成纤维细胞。研究了机械应力对HPLF-Y和HPLF细胞生长和分化的影响。在有或无视黄酸（RA）、1，25-二羟基维生素D_3（D_3）或两者同时存在的情况下，采用柔性底培养板和真空系统，以5%或10%的拉伸率对细胞施加间歇性机械应变10秒，静止10秒或50秒。在拉伸24小时后，我们检测了两种细胞的碱性磷酸酶（ALP）活性和DNA量。用0.1mM RA处理8天可抑制HPLF-Y和HPLF的DNA合成。在培养后期，RA对两种细胞生长的抑制作用均强于D_3或RA与D_3联合处理。HPLF-Y的碱性磷酸酶（ALP）活性随处理时间的延长而升高， ...更多信息用0.1mM RA处理8天后，HPLF的细胞凋亡率无明显变化。在间歇拉伸（5%，3个循环/分钟，24小时），HPLF的细胞生长受到刺激，而HPLF-Y的细胞生长受到抑制。D_3或RA与D_3联合处理的HPLF-Y细胞，在融合期施加5%的机械应力，ALF合成明显增加。生长期牵拉RA和D_3单独处理或RA和D_3联合处理HPLF，均能提高ALP活性。这些数据表明HPLF-Y和HPLF在对RA或机械应力的反应方面存在明显差异。本研究提示HPLF-Y和HPLF在牙周组织发育过程中的改建中具有不同的功能，D_3和RA能够改变HPLF和HPLF-Y细胞对机械应力的反应。少