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Production of Useful Compounds by Selective Control of Biocatalyst

通过选择性控制生物催化剂生产有用的化合物

基本信息

批准号：
05671765
负责人：
ASADA Yoshihisa
金额：
$ 1.34万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671765/
关键词：
Biocatalyst Biotransformation Plant biocatalyst Hairy root Anthocyanin 18beta-Glycyrrhetinic acid Resibufogenin

项目摘要

1. Biotransformation of 18beta-glycyrrhetinic acidThree new biotransformation products and three known compounds were isolated from the hairy root cultures of Panax ginseng following administration of 18beta-glycyrrhetinic acid. Ginseng hairy roots showed the biotransformation abilities of glycosylation and malonylation to 18beta-glycyrrhetinic acid. Two bioconversion products possess the same sweetness as glycyrrhizin.2. Biotransformation of resibufogeninOn administration of resibufogenin which is a cardio-active component of Chinese crude drug, Japanese name "Sen-so" to the hairy root cultures of Panax ginseng, four new biotransformation products were isolated. In the bioconversion of resibufogenin, glycosylation by glucose and sophorose at C-3 position and hydroxylation at C-1beta and C-5beta positions were observed. Further, marinobufagin which is 5beta-hydroxylated compound of resibufogenin was isolated from the cell suspension cultures of Eucalyptus perriniana following administration of resibufogenin.3. Anthocyanin production in cultured cells of Aralia cordata Thunb.We had selected anthocyanin high-producing strains of the cultured cells of Aralia cordata. The anthocyanin yield was 13.0% maximum dry weight on being cultured for three weeks in darkness.4. Saponin production in hairy root cultures of Astragalus membranaceusFour new and eight known astragalosides were isolated from the hairy root cultures of Astragalus membranaceus. The glycosidic fraction of A.m C-2 strain and traditional crude drug "Astragali Radix" showed the same pattern in TLC and HPLC.

1.18β-甘草次酸的生物转化从人参毛状根培养物中分离得到3个新的生物转化产物和3个已知化合物。人参毛状根具有糖基化和丙二酸化转化为18β-甘草次酸的能力。两种生物转化产品具有与甘草酸相同的甜度。利用人参毛状根培养物中的心脏活性成分--脂蟾毒配基对人参毛状根进行生物转化，共分离出四种新的生物转化产物。在脂蟾毒配基的生物转化中，观察到C-3位葡萄糖和槐糖的糖基化以及C-1β和C-5β位的羟基化。此外，从加入脂蟾毒配基后的欧洲桉树悬浮培养物中分离得到一种5β羟基化的脂蟾毒配基化合物--马利诺蟾毒配基。龙牙菜培养细胞花色苷的产生我们从龙牙菜培养细胞中筛选出花色苷高产菌株。在黑暗中培养3周，花色苷产量可达最大干重的13.0%。黄芪毛状根培养物中皂苷的产生从黄芪毛状根培养物中分离得到4个新的和8个已知的黄芪皂苷。Am C-2菌株和中药材黄芪的糖苷组分在薄层色谱和高效液相色谱中显示出相同的图谱。