The study of opportunistic infection route and prevention on children with leukemia.

儿童白血病机会性感染途径及预防研究

• 批准号: 05671942
• 负责人: DOI Matsuko
• 金额: $ 1.34万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671942/
• 关键词: Opportunistic infection; Candida yeast; Karyotype; Pulse-field gel electrophoresis; Chromosome; DNA; カンジダ酵母

Candida yeasts are occasionally recovered from patients with leukemia in spite of antifungal therapy used during chemotherapy. It is not yet known whether yeasts in these patients are of endogenous or exogenous origin. We examined the strain relatedness of Candida albicans isolated from 3 patients with leukemia (A,B,C) and their bedside parents using pulse-field gel electrophoresis (PFGE) , restriction fragment length polymorphism (RFLP) by smaI digestion and its Southern hybridization pattern by C.albicans specific probe RPS1. SmaI digestion and Southern hybridization by RPS1 showed identical or similar patterns among Candida isolates in patient A and his mother, although their karyotype were different. Isolates from patient B and both parents showed identical electrophoretic karyotype, SmaI and hybridization patterns. Since electrophoretic karyotype are more variable than RFLP and their hybridization patterns, the identity of the latter suggests a close relatedness between strains. Our results also suggest that transmission of yeast strains may have occurred between patient A and his mother, and between patient B and her parents. Isolates from patient C and her mother are thought to have originated from different strains since different patterns were obtained in electrophoretic karyotype, SmaI digestion and Southern hybridization patterns.

尽管在化疗期间使用了抗真菌治疗，但白血病患者体内的念珠菌酵母菌偶尔会恢复。目前尚不清楚这些患者中的酵母菌是内源性还是外源性。用脉冲场凝胶电泳法(PFGE)、限制性片段长度多态(RFLP)和白念珠菌特异性探针RPS1的Southern杂交技术，对3例白血病患者(A、B、C)及其床边双亲分离的白色念珠菌进行了菌株亲缘关系检测。SmaI酶切和RPS1 Southern杂交显示，患者A和其母亲分离的念珠菌虽然核型不同，但具有相同或相似的带型。患者B和双亲的分离株具有相同的电泳型、SMAI和杂交模式。由于电泳核型比RFLP及其杂交模式更具变异性，后者的一致性表明菌株之间存在密切的亲缘关系。我们的结果还表明，酵母菌株的传播可能发生在患者A和他的母亲之间，以及患者B和她的父母之间。从患者C和她的母亲分离的菌株被认为来自不同的菌株，因为在电泳核型、SMAI酶切和Southern杂交图谱中获得了不同的图谱。

项目成果

Doi Matsuko: "Strain relatedness of Candida albicans strains isolated from children with leukemia and theri bedside parents." Journal of Clinical Microbiology. 32(9). 2253-2259 (1994)

Doi Matsuko：“从白血病儿童及其床边父母身上分离出的白色念珠菌菌株的菌株相关性。”

Doi Matsuko, et al.: "Electrophoretic karyotypes of pathogenic yeasts from hospital." Bulletin of College of Medical Science Gifu University. 1. 31-36 (1994)

Doi Matsuko 等人：“医院致病性酵母菌的电泳核型”。

Doi,Matsuko: "Electrophoretic karyotypes of Candida yeasts recurrently isolated from single patients." Mycrobiol.Immunology. 38(1). 19-23 (1994)

Doi，Matsuko：“从单个患者中反复分离出念珠菌的电泳核型。”

Doi Matsuko, et al.: "Electrophoretic karyotypes of isolates of Candida albicans from hospitalized patients." Journal of Medical and Veterinary Mycology. 32. 133-140 (1994)

Doi Matsuko 等人：“住院患者白色念珠菌分离株的电泳核型”。

Doi Matsuko, et al.: "Strain relatedness of Candida albicans strains isolated from children with leukemia and their bedside parents." Journal of clinical Microbiology. 32. 2253-2259 (1994)

Doi Matsuko 等人：“从白血病儿童及其床边父母身上分离出的白色念珠菌菌株的菌株相关性。”