The study of opportunistic infection route and prevention on children with leukemia.

尽管在化疗期间使用了抗真菌治疗，但偶尔也能从白血病患者身上恢复念珠菌。目前尚不清楚这些患者体内的酵母菌是内源性的还是外源性的。采用脉冲场凝胶电泳（PFGE）、smmai酶切限制性片段长度多态性（RFLP）和白色念珠菌特异性探针RPS1的Southern杂交模式对3例白血病患者（A、B、C）及其床边父母分离的白色念珠菌进行亲缘性分析。患者A及其母亲的念珠菌分离株的smi消化和RPS1的Southern杂交显示出相同或相似的模式，尽管它们的核型不同。从患者B和双亲分离的菌株显示相同的电泳核型、SmaI和杂交模式。由于电泳核型比RFLP及其杂交模式更易变异，后者的同一性表明菌株之间的亲缘关系密切。我们的结果还表明，酵母菌株的传播可能发生在患者A和他的母亲之间，以及患者B和她的父母之间。患者C及其母亲的分离株被认为来自不同的菌株，因为在电泳核型、smi消化和Southern杂交模式上获得了不同的模式。

Candida yeasts are occasionally recovered from patients with leukemia in spite of antifungal therapy used during chemotherapy. It is not yet known whether yeasts in these patients are of endogenous or exogenous origin. We examined the strain relatedness of Candida albicans isolated from 3 patients with leukemia (A,B,C) and their bedside parents using pulse-field gel electrophoresis (PFGE) , restriction fragment length polymorphism (RFLP) by smaI digestion and its Southern hybridization pattern by C.albicans specific probe RPS1. SmaI digestion and Southern hybridization by RPS1 showed identical or similar patterns among Candida isolates in patient A and his mother, although their karyotype were different. Isolates from patient B and both parents showed identical electrophoretic karyotype, SmaI and hybridization patterns. Since electrophoretic karyotype are more variable than RFLP and their hybridization patterns, the identity of the latter suggests a close relatedness between strains. Our results also suggest that transmission of yeast strains may have occurred between patient A and his mother, and between patient B and her parents. Isolates from patient C and her mother are thought to have originated from different strains since different patterns were obtained in electrophoretic karyotype, SmaI digestion and Southern hybridization patterns.