The study of opportunistic infection by Pulsed Field Gel Electrophoresis (PFGE) on leukemia patients

本研究试图找出念珠菌在白血病患者体内的感染途径。为此目的，个别菌株的鉴定和菌株划分的澄清是至关重要的。核型，染色体分离模式，通过脉冲场凝胶电泳(PFGE)，最近已被用于医学上重要的酵母菌的流行病学研究。我们追踪了5例白血病患者的念珠菌感染情况。尽管在化疗期间服用了抗真菌药物，但在5名患者中有3名患者被分离出白色念珠菌。他们的父母在第一次检查时就出示了白色念珠菌的证据。我们不能在他们房间的空气中分离出任何白色念珠菌。从同一房间住院的患者中分离的白色念珠菌的核型相互不同。然而，从2例患者分离的白色念珠菌的某些核型与其父母中检测到的相似，表明患者和患者的白色念珠菌是相同的。在我们的调查中，患者唾液中的白色念珠菌占主导地位。所以。白念珠菌可能是通过唾液从父母传给患者的。

In this study, we tried to find out the infection route of Candida yeasts on leukemia patients. For that purpose, the identification of individual isolates and the clarification of strain delineation are essential. Karyotype, the chromosome separating pattern, by pulsed field gel electrophoresis (PFGE), has recently been used for the epidemiological study of medically important yeasts. We traced Candida infection in 5 leukemia patients. C. albicans was isolated in 3 of the 5 patients in spite of the fact that they took antifungal agents during chemotherapy. THeir parents had shown evidence of C. albicans at their first examinations. We could not isolate any C. albicans in the air of their rooms. THe Karyotypes of C. albicans isolated from indivisual patients who were hospitalized in the same room were found to be different from each other. However, some karyotypes of C. albicans isolated from 2 patients were similar to those detected in their parents, suggesting that the C. albicans from the patients and their patients were the same. In our investigation, C. albicans was dominant in the saliva of the patients. So. the C. albicans might have been transfered from the parents to the patients through saliva.