The study of opportunistic infection by Pulsed Field Gel Electrophoresis (PFGE) on leukemia patients

脉冲场凝胶电泳（PFGE）对白血病患者机会性感染的研究

• 批准号: 02671078
• 负责人: DOI Matsuko
• 金额: $ 1.41万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02671078/
• 关键词: Candida Yeast; Chromosome; Opportunistic Infection; Karyotype; Leukemia; Pulsed Field Gel Electrophoresis; DNA; 染色体DNA; パルスフィ-ルドゲル電気泳動; 感染原

In this study, we tried to find out the infection route of Candida yeasts on leukemia patients. For that purpose, the identification of individual isolates and the clarification of strain delineation are essential. Karyotype, the chromosome separating pattern, by pulsed field gel electrophoresis (PFGE), has recently been used for the epidemiological study of medically important yeasts. We traced Candida infection in 5 leukemia patients. C. albicans was isolated in 3 of the 5 patients in spite of the fact that they took antifungal agents during chemotherapy. THeir parents had shown evidence of C. albicans at their first examinations. We could not isolate any C. albicans in the air of their rooms. THe Karyotypes of C. albicans isolated from indivisual patients who were hospitalized in the same room were found to be different from each other. However, some karyotypes of C. albicans isolated from 2 patients were similar to those detected in their parents, suggesting that the C. albicans from the patients and their patients were the same. In our investigation, C. albicans was dominant in the saliva of the patients. So. the C. albicans might have been transfered from the parents to the patients through saliva.

本研究旨在探讨白血病患者念珠菌感染的途径。为此，必须鉴定单个分离株并阐明菌株划分。染色体核型，染色体分离模式，脉冲场凝胶电泳（PFGE），最近已被用于流行病学研究的医学上重要的酵母菌。我们追踪了5例白血病患者的念珠菌感染。C. 5例患者中有3例在化疗期间服用抗真菌药物，但仍分离出白色念珠菌。他们的父母已经证明了C。白色念珠菌在他们的第一次检查。我们无法分离出任何C。空气中的白色念珠菌C.的核型。同一病房的不同患者分离的白色念珠菌也不同。然而，C. 2例患者分离出的白色念珠菌与其父母分离出的白色念珠菌相似，提示白色念珠菌可能是其父母的致病菌。白念珠菌与其患者相同。在我们的调查中，C。唾液中白色念珠菌占优势。是的梭白色念珠菌可能通过唾液从父母传给患者。

项目成果

Matsuko Doi: "Estimation of Chromosome Number and Size by Pulsed Field Gel Electrophoresis (PFGE) in Medically Important Candida Yeasts."

Matsuko Doi：“通过脉冲场凝胶电泳 (PFGE) 估计医学上重要的念珠菌酵母中的染色体数量和大小。”

Matsuko Doi: "Conditions of pulse field gel electrophoresis(PFGE)to separate Candida yeast chromosomal DNAs." Journal of General Microbiology.

Matsuko Doi：“脉冲场凝胶电泳 (PFGE) 分离念珠菌酵母染色体 DNA 的条件。”

Matsuko Doi: "Estimation of Chromosome Nubmer and Size by Pulsed Field Gel Electrophoresis(PFGE)in Medically Important Candida Yeasts." Journal of Clinical Microbiology.

Matsuko Doi：“通过脉冲场凝胶电泳 (PFGE) 估计医学上重要的念珠菌酵母的染色体数量和大小。”

Matsuko Doi: "Estimation of Chromosome Number and Size by Pulsed Fieldgel Electrophoresis in Medically Important Candida Yeasts." Journal of General Microbiology.

Matsuko Doi：“通过脉冲场凝胶电泳估计具有医学重要性的念珠菌酵母的染色体数量和大小。”

土井 まつ子: "電気泳動核型による白血病患者の日和見感染に関する研究"

土井松子：“利用电泳核型分析研究白血病患者的机会性感染”