Uptake of Cardenolides from Oleander Leaves by Daphnis nerii

水蚤从夹竹桃叶中摄取强心内酯

• 批准号: 05680517
• 负责人: YAMAUCHI Tatsuo
• 金额: $ 1.28万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680517/
• 关键词: Daphnis nerii; Nerium indicum; Cardenolide; Oleandrin; Adynerin; Ursolic acid; 強心配糖体; キュウチクトウ

A Sphingidous moss, Daphnis nerii L., has settled in Okinawa Islands. Although the larvae of D.nerii feed on oleander leaves and seem to be able to take in cardenolides from oleander, the larvae appear to retain cryptic color. In order to know the pass way of the cardenolides in the worms of D.nerii, comparative analysis of the cardenolides in the final-instar larvae and their frass, and those of the original oleander leaves was attempted.Most of the cardenolide triosides in the leaves were detected as their corresponding monosides in the worm-homogenate and the frass-extract. From the frass, adynerin was obtained in the unusual high yield, along with nine cardenolide monosides, deacetyloleandrin, odoroside A,DELTA^<16>-adynerin, oleaside A,nerigoside, neriaside, 8beta-hydroxyodoroside A and 5alpha-adynerin. Showing similar HPLC patterns, slight difference between the worm-homogenate and the frass-exract was observed, e.g.the absence of DELTA^<16>-adynerin in the worms and the peak hight of oleandrin in the two sources, suggested that a small amount of cardenolides was taken and accumulated in the worms. The toxicity of the cardenolides in the worms, however, may be insufficient to prevent attack from predators, since the worms retain cryptic color. Excretion of a large amount of adynerin in the frass and roles of adynerin and ursolic acid, two major ingredients in the worms originated from oleander leaves, are to be investigated, furthermore.

本发明公开了一种鞘藓类植物，夹竹桃，定居在冲绳。虽然夹竹桃的幼虫以夹竹桃叶为食，似乎能够从夹竹桃中摄取Cardenolides，但幼虫似乎保留了神秘的颜色。为了了解腰果酚内酯在夹竹桃蠕虫体内的传递途径，对夹竹桃末龄幼虫及其粪便中的腰果酚内酯与夹竹桃叶片中的腰果酚内酯进行了比较分析，结果表明夹竹桃叶片中的腰果酚三糖苷在虫体匀浆和粪便提取物中大部分以单糖苷的形式存在。从粪中以不寻常的高产率获得了夹竹桃苷，沿着获得了9种Cardenopropylmonosides，脱乙酰夹竹桃苷，odoroside A，DELTA-β<16>-夹竹桃苷，oleaside A，nerigoside，neriaside，8 β-hydroxyodoroside A和5 α-夹竹桃苷。结果表明，虫体匀浆液与虫体提取液的HPLC图谱相似，但二者之间略有差异，如蠕虫中不含DELTA^<16>-夹竹桃苷，而夹竹桃苷的峰高较高，表明蠕虫中有少量强心甙的摄取和积累。然而，由于蠕虫保持神秘的颜色，因此蠕虫中的Cardenolides的毒性可能不足以防止捕食者的攻击。夹竹桃叶虫粪中大量的夹竹桃素的排泄以及夹竹桃素和熊果酸在夹竹桃叶虫体内的作用有待进一步研究。

项目成果

Fumiko Abe, Tatsuo Yamauchi, Kazuo Minato: "Uptake of Cardenides and Ursolic Acid from Oleander Leaves by Larvae of Daphnis nerii" Phytochemistry submitted, March, 1995.

Fumiko Abe、Tatsuo Yamauchi、Kazuo Minato：“Daphnis nerii 幼虫从夹竹桃叶中摄取卡烯化合物和熊果酸”植物化学提交，1995 年 3 月。

Fumiko Abe: "Uptake of Cardenolides and Ursolic Acid from Oleander Leaves by Larvae of Daphnis nerii" Phytochemistry. (submitted, March 1995).

Fumiko Abe：“水蚤幼虫从夹竹桃叶中摄取强心内酯和熊果酸”植物化学。