Excited state dynamics of retinal proteins by the Fourier transform of optical absorption spectra.

通过光学吸收光谱的傅立叶变换研究视网膜蛋白质的激发态动力学。

• 批准号: 05680575
• 负责人: KAKITANI Toshiaki
• 金额: $ 1.28万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680575/
• 关键词: retinal proteins; bacteriorhodopsin; optical absorption spectra; all trans retinal; time correlation function; photoisomerization; Fourier transform; excited state dynamics; 温度効果; レチナールの光異性化

1. By menas of the Fourier transform method of optical absorption spectra, we obtained the time-correlation function of vibrational wavepacket in the excited state of bacteriorhodopsin.2. We obtained the temperature dependence of the time-correlation funciton of bacteriorhodopsin. We found that a large temperature effect exists at around 30,90 and 150 fs and that no appreciable temperature effect for the other time region. We could not reproduce this temperature effect by theoretical calculations using the harmonic oscillator model.3. We made the similar analysis for the modified bacteriorhodopsin with retinal analog where 13=14 bond is locked. We observed the time-correlation funciton for this modified bacteriorhodopsin does not appreciably differ from that of the native one, except for the early 20 fs, indicating that the other bonds than 13=14 bond are twisted in the excited state dynamics.

1.利用光学吸收谱的傅里叶变换方法，得到了细菌视紫红质激发态振动波包的时间相关函数.得到了细菌视紫红质的时间相关函数随温度的变化关系。我们发现在30，90和150 fs附近存在很大的温度效应，而在其它时间区域没有明显的温度效应。利用谐振子模型进行理论计算，我们无法再现这种温度效应.我们对13=14键被锁定的视网膜类似物修饰的细菌视紫红质进行了类似的分析。我们观察到这种修饰的细菌视紫红质的时间相关函数与天然细菌视紫红质的时间相关函数没有明显的不同，除了早期的20 fs，表明除了13=14键以外的其他键在激发态动力学中是扭曲的。

项目成果

A.Wada et al.: "A New Rodopsin Analog Involving 11z-8,18-Ethanoretinal as a Chromophore." Chem. Pharm. Bull.41. 793-795 (1993)

A.Wada 等人：“一种新的视紫红质类似物，涉及 11z-8,18-Ethanoretinal 作为发色团。”

H.Imai et al.: "Direct Observation of the Thermal Equilibriam Among Limirhodopsin,Metarhodopsin I and Metarhodopsin II in Chicken Rhodopsin." Biochemistry. 33. 14351-14358 (1994)

H.Imai 等人：“鸡视紫红质中 Limirhodopsin、Metarhodopsin I 和 Metarhodopsin II 热平衡的直接观察”。

垣谷俊昭 他: "基底状態の分子の光吸収スペクトルから励起状態ダイナミクスの情報を得る方法(FTOA)の開発-ロドプシンの光異性化ダイナミクスへの応用" 生物物理, 6 (1993)

Toshiaki Kakitani 等人：“开发一种从基态分子的光学吸收光谱 (FTOA) 获取激发态动力学信息的方法 - 在视紫红质光异构化动力学中的应用”生物物理学，6 (1993)

T.Kakitani: "Frontiers of Photobiology" Elsevier Science Publishers, 6 (1993)

T.Kakitani：“光生物学前沿”Elsevier Science Publishers，6 (1993)

H.Nagae et al.: "Calculation of the Excitation Transfer Elements Between the S_2 or S_1 State of Carotenoid and the S_2 or S_1 State of Bacteriochlorophyll." J.Chem. Phys.98. 8012-8023 (1993)

H.Nagae 等人：“类胡萝卜素 S_2 或 S_1 状态与细菌叶绿素 S_2 或 S_1 状态之间的激发传递元素的计算”。