Physico-chemical Basis of Energetics and Dynamics of Membtane Proteins

由于今届是这个计划的最后一年，我们集中讨论了一些重要的具体议题，这些议题是委员们取得进展或引起很大兴趣的。之后，我们进行了全面的讨论。具体的题目是l。从与色觉相关的光感受器蛋白质的一级结构推测视色素的系统发育树。2.光诱导分子内电子转移的飞秒和皮秒动力学。3.傅里叶变换红外光谱的原理及应用。4.蛋白质基质中的电子转移路径：Kuki和Wolyness的量子力学Monte Carlo模拟和Berrarn理论的评论，通过键机制的电子隧穿矩阵元素。5.蛋白质热力学的量热法研究。6.通过光学吸收光谱的傅里叶变换的激发态动力学。在全面的讨论中，我们对本项目的活动进行了概述。L.成员特异性br ...更多信息 ane蛋白：我们参照已知的球状蛋白的性质进行了比较研究。我们对蛋白质多重折叠螺旋结构的稳定性和功能进行了研究。我们研究了膜蛋白内游离氨基酸残基的稳定性和反应性。2.能量学：我们考虑了能量学在调节蛋白质结构松弛中的作用。从能量学的角度探讨了高效的能量转换和具体的反应机理。3.动力学：我们将飞秒和皮秒光谱应用于视网膜蛋白质的光异构化动力学。.我们还发展了光学吸收谱的傅里叶变换新技术，用于研究激发态动力学。基于这些讨论，我们都深刻地认识到，从能量学和动力学的角度对膜蛋白的结构和功能的化学研究作为广泛的生命科学的一个基础领域，正变得比以前更加重要。少

since this term is the last year of this project, we focused our discussion on some important specific topics which were progressed by the members or attracted much interests. After them, we made a comprehensive discussions. The title of the specific topics are l. Phylogenetic tree of visual pigments as estimated from the primary structure of photoreceptor proteins related to color vision. 2. Femto-and picoseconds dynamics of photoinduced intramolecular electron transfer. 3. Principle and application of FTIR spectra. 4. Path of electron transfer in the protein madia : Reviews of Quantum-mechanical Monte Carlo simulation by Kuki and Wolyness and of Berrarn theory the electron tunneling matrix element by the throughbond mechanism. 5. Thermodynamics of proteins as observed by the calorimetry. 6. Excited state dynamics by the Fourier transform of the optical absorption spectrum. In the comprehensive discussions, we made survey of our activity of the present project. l. Specificity of membr … More ane proteins : We made comparative study with reference to the well-known properties of the globular proteins. We proceeded our study on the stability and function of the several times folded helical structure of proteins. We investigated the thermodynamical stability and reactivity of ionized amino icid resdues inside the membrane proteins. 2. Energetics : We considered how the energetics plays a role of regulation the relaxation of protein structures. We investigated the highly efficient energy conversion and specific reaction mechanisms from the viewpoint of the energetics. 3. Dynamics : We applied the femto-and picosecond spectroscopy to the photoisomerization dynamics of retinal proteins. . We also developed a new technique of Fourier tranform of optical absorption spectrum for the study of excited state dynamics. Based on these discussions, we all deeply recognized that the phosico-chemical study of the structure and function of membrane proteins from the standpoint of energetics and dynamics is becoming much more important than before as a basic area of the broad life science. Less