Pulsed ENDOR studies of the local structure and function of water oxidizing system in Photosynthesis.
光合作用中水氧化系统的局部结构和功能的脉冲 ENDOR 研究。
基本信息
- 批准号:06452073
- 负责人:
- 金额:$ 3.14万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1994
- 资助国家:日本
- 起止时间:1994 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The EPR line shape of tyrosine Z (Yz) radical in Tris-treated PS II has been studied by pulsed ENDOR and was found to change at lower pH below 6.5. Based on the observed hyperfine interaction parameters, the electronic state was found to be that of cation radical. This radical mediates the electron transfer from water to the reaction center P680 and its hydrogen bonds with other amino acids play an important role. Pulsed ENDOR is a powerful mean to study the local effect of hydrogen bonding. Not only the surrounding structure of Yz radical is flexible as studied by matrix ENDOR,but also the electronic structure is responsible to the reactivity of the radical.In the acceptor side of PS II the non-hem iron was depleted or Zn-substituted. ESEEM study of the acceptor quinone (Q_A^-) showed 2 nitrogen couplings with this molecule. The one is assigned to histidine and the other to a peptide nitrogen. Pulsed ENDOR has shown the existence of exchangeable protons including two hydrogen bonds. CN treatment of PS II resulted in the loss of nitrogen signal from ESEEM of Q_A^-, that was assigned to histidine, along with structural change in the hydrogen bondings.New technique called ELDOR has been applied to detect the dipolar interaction between radicals. In the donor side of Ca-depleted PS II,the distances from tyrosine D (Yd) to the trapped Yz^+, S_3 signal species, and the Mn cluster were determined to be 29,30 and 27 A respectively.
用脉冲Endor研究了Tris处理的PSⅡ中酪氨酸Z(YZ)自由基的EPR谱线形状,发现在较低的pH低于6.5时,YZ自由基的EPR线形发生了变化。根据观察到的超精细相互作用参数,发现其电子态为阳离子自由基态。该自由基介导水向反应中心P680的电子转移,其与其他氨基酸的氢键起着重要作用。脉冲Endor是研究氢键局域效应的有力手段。通过矩阵Endor研究表明,YZ自由基的周围结构是柔性的,电子结构对自由基的反应活性也有影响,在PS II的受主一侧,非杂质铁被耗尽或被锌取代。受体对苯二酚(Q_A^-)的ESEEM研究表明,该分子与2个氮偶联。一个被指定为组氨酸,另一个被指定为肽氮。脉冲Endor表明存在可交换的质子,包括两个氢键。CN处理PS II导致ESEEM中归属组氨酸的Q_A^-的氮信号丢失,氢键结构发生变化。在缺钙PS II的供体侧,酪氨酸D(Yd)到捕获的YZ+、S 3信号物种和锰团簇的距离分别为29、30和27 A。
项目成果
期刊论文数量(82)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A. V. Astashkin: "Pulsed EPR Study of Manganese g=4.1 Signal in Plant Photosystesm II" J. Magn. Reson.105B. 113-119 (1994)
A. V. Astashkin:“植物光系统 II 中锰 g=4.1 信号的脉冲 EPR 研究”J. Magn。
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Yoshio. Kodera.: "EPR study of trapped tyrosine Z^+ in Ca-depleted Photosystem II" Biochim. Biophys. Acta,. 1232. 43-51 (1995)
吉雄。
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Y.Kodera,S.A.Dzuba,H.Hara and A.Kawamori: "Distances from tyrosine D^+ to the manganese cluster and the acceptorion in Photosystem II as determined by selective hole burning in EPR spectra" Biochim.Biophys.Acta,. 1186. 91-99 (1994)
Y.Kodera、S.A.Dzuba、H.Hara 和 A.Kawamori:“通过 EPR 光谱中的选择性烧孔确定从酪氨酸 D^ 到锰簇和光系统 II 中接受离子的距离”Biochim.Biophys.Acta,。
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Sergei A.Dzuba and Asako Kawamori: "Selective Hole Burning in EPR : Spectral Diffusion and Dipolar Broadening" Concepts in Magnetic Resonance. 8. 49-61 (1996)
Sergei A.Dzuba 和 Asako Kawamori:“EPR 中的选择性烧孔:谱扩散和偶极展宽”磁共振概念。
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Sergei A. Dzuba: "Selective Hole Burning in EPR: Spectral Diffusion and Dipolar Broadening" Concepts Magne. Reson.8. 49-61 (1996)
Sergei A. Dzuba:“EPR 中的选择性烧孔:光谱扩散和偶极展宽”概念 Magne。
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KAWAMORI Asako其他文献
KAWAMORI Asako的其他文献
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{{ truncateString('KAWAMORI Asako', 18)}}的其他基金
3-dimensional configuration of the electron transfer chain in photosynthesis studied by pulsed EPR
脉冲 EPR 研究光合作用中电子传递链的 3 维构型
- 批准号:
13640412 - 财政年份:2001
- 资助金额:
$ 3.14万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Pulsed ELDOR detected NMRによるタンパク内分子の構造解析
通过脉冲 ELDOR 检测 NMR 对蛋白质内的分子进行结构分析
- 批准号:
13780494 - 财政年份:2001
- 资助金额:
$ 3.14万 - 项目类别:
Grant-in-Aid for Young Scientists (B)