Studies on Molecular Mechanism of Bioluminescence

生物发光分子机制研究

• 批准号: 06453219
• 负责人: MIKI Kunio
• 金额: $ 1.22万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06453219/
• 关键词: Bioluminescence; Luciferase; EP390; X-ray Crystal Structure; Three-dimensional Structure; Flavin; FP390

The three-dimensional structure of FP390 protein from a bioluminescent bacterium, Photobacterium phosphoreum, has been determined at 3 A resolution on the basis of diffraction data collected by synchrotron radiation. The crystal structure of this protein was solved by the molecular replacement procedure and refined at 2.7 A resolution to a R value of 24.0%.The remarkable feature of the structure is that two monomer molecules related by the non-crystallographic two-fold axis to form a dimeric structure. There two Q-flavin molecules in each monomer. One of them is located at the molecular surface and the another is at the dimer interface. The electron density of myristic acids of the Q-flavin at the dimer interface in both monomer are weak and unclear, showing the possibility that Q-flavins bound in this site are not a single species but a mixture of two components.

利用同步辐射衍射数据，以3 a分辨率测定了磷光菌（Photobacterium phosphoum） FP390蛋白的三维结构。该蛋白的晶体结构用分子置换法求解，并在2.7 A分辨率下细化到R值为24.0%。该结构的显著特点是两个单体分子通过非结晶双重轴相连形成二聚体结构。每个单体中有两个q -黄素分子。其中一个位于分子表面，另一个位于二聚体界面。在这两个单体的二聚体界面上，Q-flavin的肉豆蔻酸的电子密度都很弱且不清楚，表明在这个位点结合的Q-flavin可能不是一个单一的物种，而是两种成分的混合物。

项目成果

A. Kita: "Crystal Structure Determination of a Flavoprotein FP_<390> from a Luminescent Bacterium, Photobacterium Phosphoreum" Acta Crystallogr.D52. 77-86 (1996)J. Biochem.

A. Kita：“来自发光细菌磷光杆菌的黄素蛋白 FP_<390> 的晶体结构测定”Acta Crystallogr.D52。

A.Kita: "Crystal Structure Determination of a Flavoprotein FP_<390> from a Luminescent Bacterium, Photobacterium phosphoreum" J.Biochem.117. 575-578 (1995)

A.Kita：“来自发光细菌磷光杆菌的黄素蛋白 FP_<390> 的晶体结构测定”J.Biochem.117。

A. Kita: "Crystal Structure Determination of a Flavoprotein FP_<390> from a Luminescent Bacterium, Photobacterium Phosphoreum" Acta Crystallogr.D52. 77-86 (1996)J. Biochem.Acta Crystallogr.

A. Kita：“来自发光细菌磷光杆菌的黄素蛋白 FP_<390> 的晶体结构测定”Acta Crystallogr.D52。

A. Kita: "The Structure of Flavoprotein FP_<390> from a Luminescent Bacterium photobacterium phosphoreum at 2.7Å Resolution" J. Biochem.117. 575-578 (1995)

A. Kita：“2.7Å 分辨率下发光细菌的黄素蛋白 FP_<390> 的结构”J. Biochem.117（1995）。

A. Kita: "Crystal Structure Determination of a Flavoprotein FP_<390> from a Luminescent Bacterium, Photobacterium phosphoreum" J. Biochem.117. 575-578 (1995)

A. Kita：“来自发光细菌磷光杆菌的黄素蛋白 FP_<390> 的晶体结构测定”J. Biochem.117。