The phylogenetical relationships of four species in the family Hynobiidae from Sichuan and its neighborhood, China.

对四川及其邻区4种大鲵的系统发育关系进行了细胞遗传学和分子遗传学研究。对四川峨眉山的平chonbatrachuperus、湖北利川的Pseudohynobius flavomaculatus、山西邹之的Pseudohynobius tsinpaensis和四川武山的Ranodon shihi标本进行预处理，在四川进行细胞遗传学和分子遗传学研究，并运往日本东宝大学。从常规Giemsa染色和c带核型分析中，可以清楚地看出石氏河鼠与清华p.p insinpaensis的亲缘关系非常密切。因为这两个物种的核型几乎相同。在系统发育上，黄斑p.a romaculatus与石氏p.a rshihi和清华p.a rsinpaensis的关系比平丘b.a pinchonii更接近。由于黄颡鱼的同源染色体部分与全基因组染色体的比例大于平氏白颡鱼，分离基因组DNA，用限制性内切酶（Dra I和Scr F1）处理后，进行电泳。之后，分别以石竹和黄颡鱼的0.39 kb和0.38 kb DNA片段作为探针，进行Southern blot杂交。通过对基因组dna的电泳图谱和Southern杂交图谱的分析，支持了细胞遗传学分析的系统发育关系。

Cytogenetic and molecular genetic studies about the phylogenetic relationships of four Chinese salamanders in Sichuan and its neighborhood were performed. Specimens of Batrachuperus pinchonii, from Omei mountain, Sichuan, Pseudohynobius flavomaculatus from Lichuan, Hubei, Pseudohynobius tsinpaensis from Zouzhi, Shensi, and Ranodon shihi from Wushan, Sichuan were done with some pretreatment for cytogenetic and molecular genetic studies in Sichuan, and were transported to Toho university in Japan.From the analysis or the conventional Giemsa stained and the C-banded karyotypes, it was revealed clearly that the relationship of R.shihi and P.tsinpaensis is phylogenetically very close. Because the karyotypes of the two species are almost the same. P.flavomaculatus is phylogenetically considerably closer to R.shihi and P.tsinpaensis than B.pinchonii. Because the ratio of the homeologous chromosome parts to whole genomic chromosomes of P.flavomaculatus is more than that of B.pinchonii.After the isolation of genomic DNA and treatment with restriction endonuclease (Dra I and Scr F1), the electrophoresis was performed. After that, the Southern blot hybridization was also performed with 0.39 and 0.38 kb DNA fragments from R.shihi and P.flavomaculatus, respectively, as probes. After analyzes of the electrophoretic patterns of genomic DNAs and of the Southern blot hybridization patterns, the results of phylogenetic relationships from the cytogenetic analyzes were supported.