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The phylogenetical relationships of four species in the family Hynobiidae from Sichuan and its neighborhood, China.

中国四川及其邻近地区鳖科四种物种的系统发育关系。

基本信息

批准号：
06454032
负责人：
KOHNO Sei-ichi
金额：
$ 3.52万
依托单位：
TOHO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Cytogenetic and molecular genetic studies about the phylogenetic relationships of four Chinese salamanders in Sichuan and its neighborhood were performed. Specimens of Batrachuperus pinchonii, from Omei mountain, Sichuan, Pseudohynobius flavomaculatus from Lichuan, Hubei, Pseudohynobius tsinpaensis from Zouzhi, Shensi, and Ranodon shihi from Wushan, Sichuan were done with some pretreatment for cytogenetic and molecular genetic studies in Sichuan, and were transported to Toho university in Japan.From the analysis or the conventional Giemsa stained and the C-banded karyotypes, it was revealed clearly that the relationship of R.shihi and P.tsinpaensis is phylogenetically very close. Because the karyotypes of the two species are almost the same. P.flavomaculatus is phylogenetically considerably closer to R.shihi and P.tsinpaensis than B.pinchonii. Because the ratio of the homeologous chromosome parts to whole genomic chromosomes of P.flavomaculatus is more than that of B.pinchonii.After the isolation of genomic DNA and treatment with restriction endonuclease (Dra I and Scr F1), the electrophoresis was performed. After that, the Southern blot hybridization was also performed with 0.39 and 0.38 kb DNA fragments from R.shihi and P.flavomaculatus, respectively, as probes. After analyzes of the electrophoretic patterns of genomic DNAs and of the Southern blot hybridization patterns, the results of phylogenetic relationships from the cytogenetic analyzes were supported.

用细胞遗传学和分子遗传学的方法研究了四川及邻区4种中国火蜥蜴的系统发育关系。对采自四川峨眉山的平川水母、湖北利川的黄斑水蚤、陕西邹治的水华水蚤和四川武山水生水母的标本进行了细胞遗传学和分子遗传学的前处理，并将其送入日本东湖大学，通过常规Giemsa染色和C-带核型分析，明确了水华水母与华东水母的亲缘关系非常近。因为这两个物种的核型几乎相同。与B.pinchonii相比，黄斑扁尾轮虫在系统发育上与P.shihi和P.tsinpasis的亲缘关系更近。由于黄斑拟青霉的同源染色体部分占全基因组染色体的比例大于斑点拟青霉，提取基因组DNA，经限制性内切酶(Dra I和Scr F1)处理后，进行电泳分析。在此基础上，分别以0.39 kb和0.38 kb的DNA片段为探针进行Southern杂交。通过对基因组DNA的电泳谱和Southern杂交图谱的分析，支持了细胞遗传学分析的系统发育关系的结果。