DNA DIMINUTION (CHROMOSOME ELIMINATION) DURING DIFFERENTIATION INTO SOMATIC CELLS IN BALTIC, SOUTH PACIFIC AND NORTHEAST PACIFIC HAGFISH SPECIES.
波罗的海、南太平洋和东北太平洋海鳗物种分化为体细胞期间的 DNA 减少(染色体消除)。
基本信息
- 批准号:04640595
- 负责人:
- 金额:$ 0.19万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1992
- 资助国家:日本
- 起止时间:1992 至 1993
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Baltic hagfish, Myxine glutinosa, south Pacific hagfish, Eptatretus cirrhatus, and northeast Pacific hagfish, Eptatretus stoutii, in the order Myxinida were collected in Kristineberg Marine Biological Station in Sweden, Kaikoura Marine Station in New Zealand, and Bamfield Marine Station in Canada, respectively. M.glutinosa, E.cirrhatus, and E.stoutii have been possess different chromosome numbers in their germ cells (spermatogonia) and somatic cells (liver, blood, gill and kidney). The difference of the chromosome numbers between spermatogonia (44, 72 or 80, and 48) and somatic cells (28, 34, and 34) was 16, 38 or 46, and 14, in M.glutinosa, E.cirrhatus, and E.stoutii, respectively. In E.cirrhatus, two types of specimens, those having the smaller number of chromosomes (72) in their germ cells and those having the larger one (80) were observed. The relative amount of DNA eliminated from germ cells averaged 43.5%, (M.g.), 49.2% (E.c.type A), 52.8% (E.s.), respectively. These results clea … More rly indicate that, in the 3 hagfish species in Myxinida which live in the Baltic Sea and south Pacific Ocean and northeast Pacific Ocian, chromosome elimination takes place during the early stages of cleavage except for the ancestral germ cells.DNA from germ line cells and somatic cells in M.glutinosa, E.cirrhatus (type A, type B), E.stoutii and Paramyxine atami (Japanese hagfish) had been isolated and digested with 4 kinds of restriction enzymes. One enzyme (Dra I) generated bands limited to germ line DNA in both types of E.cirrhatus by agarose gel electrophoresis. DNA filter hybridization experiments proved that the DNA fragments, about 100 bp long, are contained only in the eliminated DNA of E.cirrhatus. These fragments are hardly contained in the germ line genomes and somatic genomes of the other three species. As a result of DNA filter hybridization by using DNA fragments (germ line-restricted EEEo1 and EEEo2 from E.okinoseanus) as probes, it was appeared that EEEo1 was detected in none of the four species and EEEo2 was done in P.atami. Less
波罗的海盲鳗、Myxine glutinosa、南太平洋盲鳗、Eptatretus cirathus 和东北太平洋盲鳗目 Myxinida 分别采集于瑞典克里斯汀伯格海洋生物站、新西兰凯库拉海洋站和加拿大班菲尔德海洋站。 M.glutinosa、E.cirathus 和 E.stoutii 在其生殖细胞(精原细胞)和体细胞(肝、血、鳃和肾)中拥有不同的染色体数目。在M.glutinosa、E.cirathus和E.stoutii中,精原细胞(44、72或80和48)与体细胞(28、34和34)之间的染色体数目差异分别为16、38或46和14。在 E.cirathus 中,观察到两种类型的样本,一种是生殖细胞中染色体数量较少(72 条)的样本,另一种是生殖细胞中染色体数量较多(80 条)的样本。从生殖细胞中消除的 DNA 相对量平均分别为 43.5% (M.g.)、49.2% (E.c.A 型)、52.8% (E.s.)。这些结果清楚地表明,在生活在波罗的海、南太平洋和东北太平洋的 3 种粘盲鳗中,除了祖先生殖细胞外,染色体消除发生在卵裂的早期阶段。来自 M.glutinosa、E.cirathus(A 型、B 型)、E.stoutii 和 Paramyxine atami 的生殖系细胞和体细胞的 DNA (日本盲鳗)已被分离并用 4 种限制性酶消化。通过琼脂糖凝胶电泳,一种酶 (Dra I) 在两种类型的 E.cirathus 中产生仅限于种系 DNA 的条带。 DNA滤膜杂交实验证明,仅在被消除的鲫鱼DNA中含有约100 bp长的DNA片段。这些片段几乎不包含在其他三个物种的种系基因组和体细胞基因组中。使用DNA片段(来自冲海棘球菌的种系限制性EEEo1和EEEo2)作为探针进行DNA滤光杂交,结果显示,在4个物种中均未检测到EEEo1,而在热海虾中检测到了EEEo2。较少的
项目成果
期刊论文数量(32)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
S.Kubota: "Germ line-restricted, highly repeated DNA sequences and their chromosomal localization in a Japanese hagfish (Eptatretus okinoseanus)." Chromosoma. 102. 163-173 (1993)
S.Kubota:“日本盲鳗(Eptatretus okinoseanus)中种系限制的、高度重复的 DNA 序列及其染色体定位。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
中井康晴、久保田宗一郎、河野晴一 他: "ニュージーランド、カナダ西海岸、並びにスウェーデン産メクラウナギの染色体放出" 染色体. II-69. 2373- (1993)
Yasuharu Nakai、Soichiro Kubota、Haruichi Kono 等人:“来自新西兰、加拿大西海岸和瑞典的盲鳗中的染色体释放”染色体 II-69。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
久保田・黒尾・河野: "ムラサキヌタウナギにおける生殖細胞に特異的なB染色体" 染色体(La Kromosomo). II-65. 2212 (1992)
Kubota、Kuroo 和 Kono:“紫色盲鳗生殖细胞特有的 B 染色体”染色体 (La Kromosomo) 2212 (1992)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
Nakai,S.,Kubota,S.,Goto,Y.,Ishibashi,T.,Davison,B.and Kohno,S.: "Chromosome elimination in Baltic,south Pacific and northeast Pacific hagfish species" (in preparation).
Nakai,S.、Kubota,S.、Goto,Y.、Ishibashi,T.、Davison,B. 和 Kohno,S.:“波罗的海、南太平洋和东北太平洋盲鳗物种的染色体消除”(准备中)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kubota,S.,Kuro-o,M.and Kohno,S.: "Analyses of germ line-restricted,highly repeated DNA sequence in Eptatretus okinoseanus II" Japanese J.Genet.67. 569 (1992)
Kubota,S.、Kuro-o,M. 和 Kohno,S.:“Eptatretus okinoseanus II 种系限制性高度重复 DNA 序列分析”日本 J.Genet.67。
- DOI:
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- 影响因子:0
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KOHNO Sei-ichi其他文献
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{{ truncateString('KOHNO Sei-ichi', 18)}}的其他基金
Fundamental studies of a developing heteromorphic sex chromosome pair in hynobiid salamanders.
小蝾螈发育中的异形性染色体对的基础研究。
- 批准号:
11833016 - 财政年份:1999
- 资助金额:
$ 0.19万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Phylogenetic Relationships of Giant Salamander Species by Means of Analyzing Genomic Highly Repetitive DNA.
通过分析基因组高度重复 DNA 来研究大鲵物种的系统发育关系。
- 批准号:
09839035 - 财政年份:1997
- 资助金额:
$ 0.19万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The phylogenetical relationships of four species in the family Hynobiidae from Sichuan and its neighborhood, China.
中国四川及其邻近地区鳖科四种物种的系统发育关系。
- 批准号:
06454032 - 财政年份:1994
- 资助金额:
$ 0.19万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
FUNDAMENTAL STUDIES OF DNA DIMINUTION (CHROMOSOME ELIMINATION) IN LOWER FISH DURING DIFFERENTIATION INTO SOMATIC CELLS.
低等鱼类分化成体细胞过程中 DNA 减少(染色体消除)的基础研究。
- 批准号:
63540508 - 财政年份:1988
- 资助金额:
$ 0.19万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
FUNDAMENTAL STUDIES OF DNA DIMINUTION (CHROMOSOME ELIMINATION) IN LOWER FISH DURING DIFFERENTIATION INTO SOMATIC CELLS.
低等鱼类分化成体细胞过程中 DNA 减少(染色体消除)的基础研究。
- 批准号:
63540508 - 财政年份:1988
- 资助金额:
$ 0.19万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)