权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular analysis of gene-products associated with self-incompatibility in Ipomoea trifida

三裂蕹自交不亲和性相关基因产物的分子分析

基本信息

批准号：
06454043
负责人：
KOWYAMA Yasuo
金额：
$ 4.42万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

The present study was performed to reveal stigma proteins associated with self-incompatibility in a wild relative of the sweet potato, Ipomoea trifida. A cDNA clone, IRK1 isolated from stigama cDNA library of I.trifida was found to encode SLG/SRK-like proteins that have been identified as self-incompatibility gene (S-gene) products in Brassica. RFLP analysis of F1 population segregating for S-alleles, however, showed that the IRK1 gene is genetically unlinked to the S-locus. S-RNases have been shown to be involved in self-incompatibility reaction of solanaceous plants. An RNase cDNA clone, IRN3 isolated from stigma cDNA library of I.trifida, also showed no linkage to the S-locus. These results suggest that Ipomoea plants possessaself-incompatibility mechanism different from those of Brassica and solanaceous plants. In the present study, stigma extracts obtained from six S-allele homozygotes were analyzed by two-dimensional electrophoresis. From comparison of electrophoretic patterns among the homozygotes, we have identified the protein spots specific for S-alleles. These S-allele specific proteins have a range of molecular mass of 60-80 kD with acidic to neutral PI.Co-segregation of these proteins with S-genotypes was confirmed in heterozygous plants for S-alleles. Further studies on the structure and function of these protein are now conducted through getting the data on the N-terminal amino acid sequence and screening of full cDNA clone from the stigma cDNA library.

本研究旨在揭示甘薯野生近缘种三裂叶牵牛（Ipomoea trifida）柱头蛋白与自交不亲和性的关系。从三裂叶鸢尾柱头cDNA文库中克隆到一个IRK 1 cDNA克隆，该克隆编码SLG/SRK类蛋白，已被鉴定为芸苔属植物自交不亲和基因（S-基因）产物。F1群体分离的S-等位基因的RFLP分析，但是，IRK 1基因是遗传不连锁的S-位点。S-RNases参与茄科植物的自交不亲和反应。从三裂叶鸢尾柱头cDNA文库中分离到的一个RNA酶cDNA克隆IRN 3也与S位点不连锁。这些结果表明，番薯属植物具有不同于芸苔属和茄科植物的自交不亲和机制。采用双向电泳技术对6个S等位基因纯合子的柱头提取物进行了分析。从纯合子之间的电泳图谱的比较，我们已经确定了S-等位基因特异性的蛋白质点。这些S-等位基因特异性蛋白质的分子量范围为60-80 kD，具有酸性至中性的PI。这些蛋白质与S-基因型的共分离在S-等位基因杂合植物中得到证实。通过对柱头cDNA文库中N端氨基酸序列的测定和全cDNA克隆的筛选，对这些蛋白的结构和功能进行了进一步的研究。