Total Internal Reflection Fluorescence Microscope (TIRF)

全内反射荧光显微镜 (TIRF)

• 批准号: 433522645
• 金额: --
• 依托单位: Ruhr-Universität Bochum
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/433522645?language=en
• 关键词: Total; Internal; Reflection; Fluorescence; Microscope

Cellular membranes separate cells from the environment and allow for compartmentalization of different cellular processes inside cells. A detailed understanding of biological membranes requires analysis of the localization and dynamics of molecules and processes in a live cell and on isolated molecular systems under well-defined incubation conditions with high time resolution. Compared to conventional microscopy, total internal reflection fluorescence microscopy in combination provides higher-contrast images of fluorophore-labelled molecules near the plasma membrane, on membrane preparations and on immobilized membrane proteins. Simultaneous detection of multiple fluorescences allows for a detailed characterization of membrane processes, which is not achievable with the current microscope setups at the Ruhr University Bochum. Implementation of this microscope will be instrumental to stimulate the scientific exchange, to increase the number of joined research projects and to improve acquisition of third-party funds.

细胞膜将细胞与环境分开，并允许划分细胞内不同的细胞过程。对生物膜的详细了解需要在明确的孵化条件下以高时间分辨率分析活细胞和分离分子系统中分子和过程的定位和动态。与传统显微镜相比，全内反射荧光显微镜组合可提供质膜附近、膜制备物和固定膜蛋白上荧光团标记分子的更高对比度图像。同时检测多种荧光可以对膜过程进行详细表征，这是波鸿鲁尔大学当前的显微镜设置无法实现的。该显微镜的实施将有助于促进科学交流、增加参与研究项目的数量以及改善第三方资金的获取。