Regulation of IgE production by the high affinity receptor for IgE (FcepsilonRI).

IgE 高亲和力受体 (FcepsilonRI) 调节 IgE 的产生。

• 批准号: 06454224
• 负责人: RA Chisei
• 金额: $ 4.42万
• 依托单位: Juntendo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454224/
• 关键词: FcepsilonRI; IgE; Mast cell; Allergy; CD40-L; B cell; IL-4; B細胞; インターロイキン4; IgE産生; クラススウィッチ

A recombinant soluble form of the alpha subunit of the human high-affinity receptor for IgE (rsFcepsilonRIalpha), one of the potent IgE-binding molecules, was tested for its ability to regulate IL-4-induced IgE synthesis by human lymphocytes. Addition of rsFcepsilonRIalpha to cultures induced a does-dependent inhibition of the T cell-dependent and independent synthesis of IgE.The suppression of IgE synthesis was observed at the protein and the mRNA levels, and it was IgE class specific. By flow cytometry, specific binding of rsFcepsilonRIalpha was detected on surface IgE-bearing B cells as well as on U266 cells, and it was completely blocked by preincubation with IgE.rsFcepsilonRIalpha bound to the cell surface IgE could be effectively dissociated not only by a large excess of IgE, but also by an anti-rsFcepsilonRIalpha mAb that competes with IgE for the binding to rsFcepsilonRIalpha. This mAb abolished the rsFcepsilonRIalpha-mediated suppression of IgE synthesis. These data suggest that rsFcepsilonRIalpha may have a function in selectively suppressing IgE synthesis through its interaction with the membrane-bound form of IgE.

人IgE高亲和受体(rsfcepsilonriα) α亚基的重组可溶性形式，是有效的IgE结合分子之一，被测试其调节il -4诱导的人淋巴细胞合成IgE的能力。在培养物中添加rsfcepsilonriα诱导了T细胞依赖性和独立合成IgE的依赖性抑制。在蛋白和mRNA水平上观察到对IgE合成的抑制，且具有IgE类特异性。流式细胞术检测到rsfcepsilonriα在表面携带IgE的B细胞和U266细胞上特异性结合，并经IgE预孵育完全阻断。结合到细胞表面的rsfcepsilonriα不仅可以被大量过量的IgE有效解离，而且还可以被抗rsfcepsilonriα单抗与IgE竞争与rsfcepsilonriα的结合。该单抗消除了rsfcepsilonriα介导的IgE合成抑制。这些数据表明，rsfcepsilonriα可能通过与膜结合形式的IgE相互作用，具有选择性抑制IgE合成的功能。

项目成果

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K.Saito.：“Fc 受体 γ 链同二聚体与 IgA 受体的物理关联。”J.Aller.Clin.Immunol.96 (1995)。

K.Naito: "Soluble form of the human high affinity receptor for IgE inhibits recurrent allergic reaction in a novel mouse model of type I allergy." Eur.J.Immunol.,. 25. 1631-1637 (1995)

K.Naito：“可溶形式的人类 IgE 高亲和力受体可抑制新型 I 型过敏小鼠模型中的复发性过敏反应。”

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Ra,C.：“肥大细胞和嗜碱性粒细胞分化和功能的生物学和分子方面。”