Alterations of opoptosis and cell cycle resulation induced by heptatitis Cvirus

丙型肝炎病毒诱导的细胞凋亡和细胞周期改变的改变

• 批准号: 06454257
• 负责人: KANEKO Yoshiyasu
• 金额: $ 2.43万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454257/
• 关键词: hepatitis virus; fomor sapressor gene; cyclin; apoptosis; アポトシス

The mechanism of hepatoma evelopment is not known, HCV has no cancer genes and the expression of HCV gene is supposed to alter the cellular mechanisms such as cell cycle regulation and induction of apoptosis. To investigate the mechanism of the action of HCV protein on these cell reguratory system, we attempted to introduce fragments of HCV gene into expression vectors. The HCV gene were reverse transcribed and amplified. Resulting gene fragments were introduced into a retroviral expression vector (pZIPneo). The retroviral vector carrying HCV gene fragments was tansfected into PA317 packaging cells. Then, the retrovirus produced by these cells were transduced into human hepatoma cells. The expression of HCV proteins are analyzed by immuno-blotting and immunofluorescent antibody technique. The studies about the effects of the HCV gene expression on the drug-induced apoptosis and cell cycle progression are in progress.

HCV在肝癌发生发展中的作用机制尚不清楚，HCV本身没有致癌基因，HCV基因的表达可能改变细胞周期调控和诱导细胞凋亡等细胞机制。为了研究HCV蛋白对这些细胞调节系统的作用机制，我们尝试将HCV基因片段导入表达载体。逆转录和扩增HCV基因。将所得基因片段导入逆转录病毒表达载体（pZIPneo）中。将携带HCV基因片段的逆转录病毒载体转染PA317包装细胞。然后，将这些细胞产生的逆转录病毒转导到人肝癌细胞中。用免疫印迹和免疫荧光抗体技术分析HCV蛋白的表达。HCV基因表达对药物诱导的细胞凋亡和细胞周期进程的影响的研究正在进行中。

项目成果

Kaneko Y.: "Apoprosis and p53 protein expression in human hepatoma cells induced by etoposide,mitomycinc and thapsigdrgin" Int.Hepat.Commun.2. 305-309 (1994)

Kaneko Y.：“依托泊苷、丝裂霉素和 thapsigdrgin 诱导人肝癌细胞凋亡和 p53 蛋白表达”Int.Hepat.Commun.2。

Tsukamoto, A,and Kaneko Y: "Alterations of unclcar P53 protein and PCNA during anticancer aray induced apoptosis of human hepdtomd cells A flow cytometric analysis" Int Hepatol Commun. 4. 88-93 (1995)

Tsukamoto, A 和 Kaneko Y：“抗癌阵列中 Unclcar P53 蛋白和 PCNA 的改变诱导人肝癌细胞凋亡，流式细胞术分析”Int Hepatol Commun。

金子義保: "肝硬変" 医薬ジャーナル. (in press). (1995)

Yoshiyasu Kaneko：“肝硬化”制药杂志（1995 年）。

Kanako Y,and Tsakamoto, A: "Apoptosis and p53 protein expression on human hepatoma cells mduced by etoposide, mitomycn C and thapsigargin" Int Hepat Commun. 2. 305-309 (1994)

Kanako Y 和 Tsakamoto, A：“依托泊苷、丝裂霉素 C 和毒胡萝卜素诱导的人肝癌细胞凋亡和 p53 蛋白表达”Int Hepat Commun。

Kaneko Y.: "Gene therapy of bepatcmd : Bysteter effect and non-apoptotic cell dearh naduced by thymidine Wilse and gemciclor"

Kaneko Y.：“bepatcmd 的基因治疗：胸苷 Wilse 和 gemcicllor 引起的 Bysteter 效应和非凋亡细胞死亡”