ESTABLISHMENT OF IMMUNOSUPPRRESIVE METHOD FOR CLINICAL XENOTRANSPLANTATION

A.大坂大学动物研究中心使用非人灵长类动物的指导方针由于非人灵长类动物非常罕见，而且与人类相似，因此将非人灵长类动物作为人类来照顾是非常重要的。B.狒狒和猪的免疫学评价1）抗猪抗体测定法的建立（通过滴定法测定红细胞，通过ELISA测定内皮细胞或白细胞）2）狒狒淋巴细胞与猪淋巴细胞的混合淋巴细胞培养（MLC）3）使用抗人淋巴细胞测定狒狒淋巴细胞亚群

A.Guideline for use of non-human primates in Osaka University Animal Research CenterAs non-human primates are rare and similar to human, it is important to take care of non-human primates as human. We made guidelines for use of non-human primate which was approved in Osaka University Animal Research Committee.B.Immunological Assessment of Baboon and Pigs1)Establishment of assay of anti-pig antibody (against red blood cells by titration method and against endothelial cells or leukocytes by ELISA)2)Mixed lymphocyte culture (MLC) of baboon lymphocytes against pig lymphocytes3)Assay of subpopulation of baboon lymphocytes using anti-human lymphocytes