DEVELOPMENT OF EGFR ANTISENSE GENE THERAPY AGAINST MALIGNANT GLIOMAS USING A RECOMBINANT RETRO-VIRUS VECTOR

表皮生长因子受体（EGFR）在胶质瘤的恶性进展中起重要作用。我们用脂质体Lipofectin^R（BRL）包裹EGFR反义寡核苷酸（正常磷酸化寡核苷酸），研究其对恶性胶质瘤细胞系的生长抑制作用，并取得了良好的效果。本研究成功地构建了表达EGFR反义寡核苷酸的重组逆转录病毒载体，并利用该逆转录病毒载体对恶性胶质瘤细胞系进行了生长抑制，对80例胶质瘤进行了EGFR结构分析，发现40%的恶性胶质瘤表达EGFR异常，并发现两种新的EGFR异常。

Epidermal growth factor receptor (EGFR) plays an important role in the progression of malignancy in gliomas. We studied the growth inhibition of the malignant glioma cell lines using an antisense EGFR oligonucleotide (normal phosphodister oligonucleotide) enveloped with Lipofectin^R (BRL) and we could have a good result. This time, we succeeded to develop the recombinant retro-virus vector expressiog EGFR antisense oligonucleotide, and we could inhibit the growth of the malignant glioma cell line using this retro-virus vector.We analyzed structure of EGFR in 80 gliomas, and found that 40% of human malignant gliomas in vivo expressed aberrant EGFR.Furthermore, we have found two kinds of new aberrant EGFR.