DEVELOPMENT OF EGFR ANTISENSE GENE THERAPY AGAINST MALIGNANT GLIOMAS USING A RECOMBINANT RETRO-VIRUS VECTOR

使用重组逆转录病毒载体开发针对恶性神经胶质瘤的 EGFR 反义基因疗法

• 批准号: 06454421
• 负责人: UEDA Satoshi
• 金额: $ 4.67万
• 依托单位: KYOTO PREFECTURAL UNIVERSITY OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454421/
• 关键词: malignant glioma; EGFR antisense gene therapy; retro-virus; aberrant EGFR; malignant afioma; EGFR ontisense gene therapy; レトロウイルス; 異常EGFR; antisense gene therapy; EGFR; retrovirus

Epidermal growth factor receptor (EGFR) plays an important role in the progression of malignancy in gliomas. We studied the growth inhibition of the malignant glioma cell lines using an antisense EGFR oligonucleotide (normal phosphodister oligonucleotide) enveloped with Lipofectin^R (BRL) and we could have a good result. This time, we succeeded to develop the recombinant retro-virus vector expressiog EGFR antisense oligonucleotide, and we could inhibit the growth of the malignant glioma cell line using this retro-virus vector.We analyzed structure of EGFR in 80 gliomas, and found that 40% of human malignant gliomas in vivo expressed aberrant EGFR.Furthermore, we have found two kinds of new aberrant EGFR.

表皮生长因子受体(EGFR)在脑胶质瘤的恶性进展中起重要作用。我们用脂联素R(BRL)包裹的反义EGFR寡核苷酸(正常磷脂寡核苷酸)对恶性胶质瘤细胞株进行了生长抑制实验，取得了较好的效果。此次我们成功构建了表达EGFR反义寡核苷酸的重组逆转录病毒载体，并利用该逆转录病毒载体抑制恶性胶质瘤细胞系的生长，分析了80例人脑胶质瘤中EGFR的结构，发现体内40%的恶性胶质瘤表达异常的EGFR。

项目成果

Noriaki Sugawa,et al: "Brain Tomar Research and Therapy" Springer-Verlag Tokyo, 4 (1995)

Noriaki Sukawa 等人：“Brain Tomar Research and Therapy” Springer-Verlag Tokyo，4 (1995)

須川典亮,上田聖: "アンチセンスDNAを用いた遺伝子治療" Clinical neuroscience. 12(6). 684-686 (1994)

Noriaki Sukawa、Kiyoshi Ueda：“使用反义 DNA 的基因治疗”临床神经科学 12(6) (1994)。

Noriaki Sugawa, Satoshi Ueda: "Gene therapy using antisense DNA" Clinical Neuroscience. 12(6). 684-686 (1994)

Noriaki Sukawa、Satoshi Ueda：“使用反义 DNA 的基因治疗”临床神经科学。

Noriaki Sugawa, Yoshio Nakagawa, Satoshi Ueda: "Point mutations of Epidermal Growth Factor Receptor Transcripts in Primary Human Malignant Gliomas" Brain Tumor Research and Therapy. Springer-Verlag, Tokyo. 233-236 (1995)

Noriaki Sukawa、Yoshio Nakakawa、Satoshi Ueda：“原发性人类恶性胶质瘤中表皮生长因子受体转录物的点突变”脑肿瘤研究与治疗。

Noriaki Sugawa,Yoshio Nakagawa,et al: "Brain Tumor Research and Therapy" Sprimger-Uelag Tokyo, 4 (1995)

Noriaki Sukawa、Yoshio Nakakawa 等人：“脑肿瘤研究与治疗”Sprimger-Uelag Tokyo，4 (1995)