Basic Research on Effects of Annexin V on Carcinogenesis, Blood Coagulation and Ovarian Function

膜联蛋白V对癌变、凝血及卵巢功能影响的基础研究

• 批准号: 06454463
• 负责人: TANAKA Toshinobu
• 金额: $ 4.42万
• 依托单位: AKITA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454463/
• 关键词: Annexin V; Carcinogenesis; Platelet aggregation; Follicular maturation; Annexin V mRNA; Annexin V; calphobindin; 組織トロンボプラスチン; 血小板

(1)We examined the level of production of CPB I in uterine cancer cells. Immunohistochemical analysis, northern blot, and in situ hybridization showed that the production of CPB I was markedly suppressed at the level transcription in both cervical and endometrial carcinoma cells when compared to their normal counterparts. Decrease in production of CPB I may lead to dysregulated activation of protein kinase C and, accordingly, may be involved in a disorder of cell differentiation, proliferation, and carcinogenesis.(Ref 1)(2)We demonstrated the localization and expression of annexin V in the reproductive organ, such as ovary, endometrium and fallopian tube. Signal of annexin V was strongly expressed in the follicular epitheliums of mature follicles and oocytes, which suggested an important role of annexin V during the process of follicular growth and oocyte maturation.(Ref 2)(3)Existence of annexin V in platelet was firstly demonstrated by Western blot. Concentration of annexin V in platelet before the aggregation lowered significantry after the reaction. It is suggested that annexin V may play an important role of the regulation of platelet aggregation.(Ref 3)

(1)我们检测了子宫癌细胞中CPB I的产生水平。免疫组织化学分析、northern blot和原位杂交显示，与正常细胞相比，宫颈和子宫内膜癌细胞中CPB I的产生在转录水平上明显受到抑制。CPB I的产生减少可能导致蛋白激酶C的激活失调，因此可能参与细胞分化、增殖和癌变的紊乱。（Ref 1）(2)我们证实了膜联蛋白V在生殖器官，如卵巢、子宫内膜和输卵管中的定位和表达。膜联蛋白V的信号在成熟卵泡和卵母细胞的卵泡上皮中强烈表达，提示膜联蛋白V在卵泡生长和卵母细胞成熟过程中发挥重要作用。（Ref 2）(3) Western blot首次证实血小板中存在膜联蛋白V。反应后血小板聚集前膜联蛋白V浓度显著降低。提示膜联蛋白V可能在血小板聚集的调控中起重要作用。(Ref 3)

项目成果

AKIHIRO KARUBE: "Suppression of Calphobindi I (CPBI) Production in Carcinoma of Uterine Cervix and Endometrium" Gynecoogic Oncdogy. 58. 295-300 (1995)

AKIHIRO KARUBE：“子宫颈癌和子宫内膜癌中 Calphobindi I (CPBI) 产生的抑制”妇科肿瘤学。

設楽芳宏: "胎盤Calphobindinと血液凝固制御" 日本産婦人科・新生児・血液学会誌. 14. S9-S10 (1994)

Yoshihiro Shitara：“胎盘钙结合蛋白和凝血控制”日本妇产科、新生儿医学和血液学会杂志14.S9-S10(1994)。

YOSHIHIRO SHIDARA: "Study of Calphobindin (human placental anticongulant protein)" Clinical Gynecology and Obstetries. Vol49. 1307-1317 (1995)

YOSHIHIRO SHIDARA：“钙结合蛋白（人胎盘抗凝血蛋白）的研究”临床妇产科。

Shidara.Y.: "Expression of annexin V mRNA in female reproductire organs" IIIrd Sappore International Symposium on ovarian Function. in press. in press

Shidara.Y.：“女性生殖器官中膜联蛋白 V mRNA 的表达”第三届札幌国际卵巢功能研讨会。

YOSHIHIRO SHIDARA: "Expression of Annexin V in Female Reproductive Organs" Frontiers in Endocrindogy. Vol.13. 15-20 (1995)

YOSHIHIRO SHIDARA：“女性生殖器官中膜联蛋白 V 的表达”内分泌学前沿。