Very Low Density Lipoprotein and Low Density Lipoprotein Receptor mRNA Expression in Placentae from Preeclamptic Pregnancies and Fetal Growth Restriction

先兆子痫妊娠和胎儿生长受限胎盘中极低密度脂蛋白和低密度脂蛋白受体 mRNA 表达

• 批准号: 10671511
• 负责人: TANAKA Toshinobu
• 金额: $ 2.37万
• 依托单位: Akita Univ.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671511/
• 关键词: Toxemia, Preeclampsia; Intrauterine Growth Returdation, Fetal Growth Restriction; Lipid Metabolism; Very Low Density Lipoprotein

The transport and-metabolic pathway of triglycerides (TG) in the placenta have not been studied in detail. The present study examined the characteristics of blood lipids in patients with Preeclampsia and gestational diabetes mellitus (GDM) and lipid metabolism in the fetoplacental system in these patients. Especially, this study measured the expressions of very low-density lipoprotein (VLDL) receptor mRNA and low-density lipoprotein (LDL) receptor mRNA in the placenta, aiming to elucidate the relationship between these mRNA expressions and the pathology. Informed consent was obtained from women (normal pregnant women and those complication) who delivered at our department. Serum levels of lipids and lipoproteins were measured. In addition, villous tissue was obtained from human placenta, and used as materials in the following experiments. RNA was extracted by the AGPC method, and Northern blot was performed. To compare the relative quantities of mRNA expression, the bands of mRNA expre … More ssion were scanned and quantitated. At delivery in the Preeclampsia and GDM groups, TG, VLDL levels were significantly higher than the levels in the normal pregnancy group. The VLDL receptor mRNA was significantly higher in the GDM group, lower in the Preeclampsia group. No significance difference in the LDL receptor mRNA was observed between the normal control, GDM and Preeclampsia. In all pregnancy groups, a positive correlation was Observed between the VLDL receptor mRNA expression and the TG or VLDL levels in umbilical blood. In the GDM group, three neonates with birth weights over 3500 g had high concentrations of TG and VLDL in the umbilical blood. At 1 month postpartum, although the blood glucose had normalized in a kind of Preeclampsia and GDM groups, serum TG and VLDL levels remained elevated, and high-density lipoprotein-cholesterol level was significantly lowered. The present study demonstrated for the first time that compared to the normal pregnant women, patients with Preeclampsia and GDM had (1)excessive lipids, especially TG, in the blood, which are the energy source in the placenta. (2) showed up-regulation of the expression of VLDL receptor mRNA in the placenta, (3) had elevated TG and VLDL levels in the umbilical blood, and (4) continued to have hyperlipemia even one month after parturition. The reason is speculated as follows. TG is necessary to compensate for impaired glucose utilization in cells because of insulin resistance, and VLDL metabolism is augmented for the transport of TG. Less

甘油三酯（TG）在胎盘中的转运和代谢途径尚未详细研究。本研究探讨了子痫前期合并妊娠期糖尿病（GDM）患者血脂的特点及其胎儿胎盘系统的脂质代谢。本研究检测了极低密度脂蛋白（VLDL）受体mRNA和低密度脂蛋白（LDL）受体mRNA在胎盘组织中的表达，旨在探讨VLDL受体mRNA和LDL受体mRNA的表达与胎盘病理的关系。在我科分娩的妇女（正常妊娠妇女和并发症妇女）获得知情同意书。测定血清脂质和脂蛋白水平。此外，从人胎盘获得绒毛组织，并用作以下实验中的材料。用AGPC法提取RNA，进行北方杂交。为了比较mRNA表达的相对量，mRNA表达的条带与mRNA表达的相对量之间存在差异。 ...更多信息 扫描并定量。子痫前期组和GDM组分娩时TG、VLDL水平均显著高于正常妊娠组。VLDL受体mRNA在GDM组明显升高，在子痫前期组明显降低。正常对照组、妊娠期糖尿病组和子痫前期组的LDL受体mRNA表达无显著性差异。在所有妊娠组中，VLDL受体mRNA表达与脐血TG和VLDL水平呈正相关。在GDM组中，3名出生体重超过3500 g的新生儿在脐带血中具有高浓度的TG和VLDL。在产后1个月时，一种先兆子痫组和GDM组的血糖虽已恢复正常，但血清TG和VLDL水平仍明显升高，高密度脂蛋白胆固醇水平明显降低。本研究首次证明，与正常孕妇相比，先兆子痫和GDM患者（1）血液中脂质（尤其是TG）过量，而脂质是胎盘中的能量来源。(2)结果显示：（1）胎盘中VLDL受体mRNA表达上调;（2）脐带血中TG和VLDL水平升高;（3）分娩后1个月仍有高脂血症。原因推测如下。由于胰岛素抵抗，TG是补偿细胞中受损的葡萄糖利用所必需的，并且VLDL代谢对于TG的运输是增强的。少

项目成果

Murata M, Tamura A, Kodama H, Hirano H, Takahashi O, Tanaka T: "Possible involvement of very low density lipoproteins in steroidogenesis in the human ovary"Mol.Hum.Reprod.. 4. 797-801 (1998)

Murata M、Tamura A、Kodama H、Hirano H、Takahashi O、Tanaka T：“极低密度脂蛋白可能参与人类卵巢的类固醇生成”Mol.Hum.Reprod.. 4. 797-801 (1998)

Murata M, Fukuda J, Kodama H, Yasuda K, Takahashi O, Ota H, Tanaka T: "Involvement of very low density lipoprotein (VLDL) and VLDL receptor in steroidgenesis in the human ovary."Front.Endoclinol.. 21. 265-272 (1999)

Murata M、Fukuda J、Kodama H、Yasuda K、Takahashi O、Ota H、Tanaka T：“极低密度脂蛋白 (VLDL) 和 VLDL 受体参与人类卵巢类固醇生成。”Front.Endoclinol.. 21. 265

村田昌功,児玉英也,後藤薫,平野秀人,田中俊誠: "妊娠中毒症および高脂血症合併妊娠症例の胎盤における超低比重および低比重リポ蛋白受容体mRNAの発現"日本妊娠中毒症学会雑誌. 5. 117-118 (1997)

Masatoshi Murata、Hideya Kodama、Kaoru Goto、Hideto Hirano、Tonsei Tanaka：“妊娠合并先兆子痫和高脂血症的胎盘中极低密度和低密度脂蛋白受体 mRNA 的表达”日本成瘾学会的日本妊娠杂志。 5. 117-118（1997）

安田師仁,村田昌功,小川正樹,福田淳,田中俊誠他: "妊娠中毒症の産褥期の脂質代謝についての検討"日本産科婦人科栄養・代謝研究会誌. 6. 58-59 (2000)

Morohito Yasuda、Masatoshi Murata、Masaki Okawa、Jun Fukuda、Tonsei Tanaka 等：“子痫前期产后脂质代谢的研究”日本妇产科学会营养与代谢杂志 6. 58-59（ 2000））

Murata M,Tamura A,Kodama H,Hirano H,Takahashi O,Tanaka T: "Possible involvement of Very low density lipoproteins in steroidogenesis in human ovary"Mol Hum Reprod. 4. 797-801 (1998)

Murata M、Tamura A、Kodama H、Hirano H、Takahashi O、Tanaka T：“极低密度脂蛋白可能参与人类卵巢类固醇生成”Mol Hum Reprod。