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Effects of neuropeptides on alveolar bone remodeling : Experimental study using Immunohistochemical method

神经肽对牙槽骨重塑的影响：免疫组织化学方法的实验研究

基本信息

批准号：
06454582
负责人：
HANADA Kooji
金额：
$ 3.71万
依托单位：
Niigata University, School of Dentistry
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Calcitonin gene-related peptide (CGRP) is one of the neuropeptides consisting of 37 amino acids, and has multiple functions such as vasodilator or neuromodulator, as well as neurotransmitter in pain sensation. First in thisproject, responses of CGRP immunopositive neurons in the trigeminal ganglionduring experimental tooth movement was examined by means of immunohistochemical method for CGRP.This study was also undertaken to determine influences of orthodontic tooth movement on CGRP stainng intensity in alveolar bone osteoblasts surrounding the peridontal ligament tension site in vivo, and to investigate initial effects of CGRP on mouse osteoblast-like (MC3T3-E1) cells applied continuous force in vitro.The result sobtained in this project were as follows :1.CGRP contained in the neurons were decreased transiently after tooth movement, and suggested that CGRP in neurons of the trigeminal ganglion appeared to be involved in pain and phenomena of periodontal tissue remodeling induced by orthodontic force.2.CGRP could be bound to osteoblasts in vivo, and orthodontic forces might alter the level of CGRP staining intensity in these cells. Dark osteoblastic staining for CGRP at time periods when osteoblastic area was relatively large, suggested that CGRP might participate in mediatingthe effects of orthodontic forces on alveolar bone osteoblasts in vivo.3.Increase in both ALPase activity and cAMP production in MC3T3-E1 cells in the presence of CGRP might support the previous report that MC3T3-E1 cells were equipped with receptors for CGRP.Especially, significant increase in camp production in continuously stressed MC3T3-E1 cells by an addition of CGRP was recognized at a short period of time after force application, suggesting that CGRP might have additional effects on the cellular responses ofmechanically stressed MC3T3-E1 cells.

降钙素基因相关肽（Calcitonin gene-related peptide，CGRP）是由37个氨基酸组成的神经肽之一，具有血管扩张或神经调质等多种功能，同时也是痛觉神经递质。本课题首先采用免疫组织化学方法检测了实验性牙齿移动过程中三叉神经节内CGRP免疫阳性神经元的反应，并在活体内观察了正畸牙齿移动对牙周膜张力区周围牙槽骨成骨细胞CGRP染色强度的影响，研究CGRP对小鼠成骨样细胞增殖的影响，体外对MC 3 T3-E1细胞施加持续力，结果如下：1.牙齿移动后，神经元内CGRP含量短暂下降，提示三叉神经节神经元内CGRP可能参与正畸力引起的疼痛和牙周组织改建现象。2. CGRP在体内可与成骨细胞结合，正畸力可改变这些细胞中CGRP的染色强度。在成骨细胞面积较大的时间段，成骨细胞对CGRP呈深染，提示CGRP可能参与介导正畸力对牙槽骨成骨细胞的影响。3.在CGRP存在的情况下，MC 3 T3-E1细胞的ALP活性和cAMP产生增加，这可能支持了先前的报道，即MC 3 T3-E1细胞具有CGRP受体。在持续应激的MC 3 T3-E1细胞中，加入CGRP在施加力后的短时间内显著增加cAMP的产生，这表明CGRP可能对机械应激的MC 3 T3-E1细胞的细胞反应具有额外的影响。