葉緑体・ミトコンドリアゲノムBAC作成とオルガネラゲノム改変新技法の確立

建立叶绿体/线粒体基因组 BAC 和修饰细胞器基因组的新技术

• 批准号: 15651085
• 负责人: 多田 光宏
• 金额: $ 2.05万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Exploratory Research
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15651085/
• 关键词: cell organella; mitochondria genome; chroloplast genome; bac vector; genome modification

本研究ではミトコンドリアや葉緑体に存在する環状DNAゲノムに対し,これらのオルガネラに遺伝子を導入もしくは改変する新規の方法を開発することを目的とする.研究期間内に以下のような成果を得た.1)ミトコンドリアおよび葉緑体へベクターを直接導入するため,マイクロマニュピレータにて電極をいれた微小管を細胞に接触もしくは刺入し,電気穿孔法にて遺伝子担体を膜を通過させてオルガネラ中に導入する方法の検討を行った.その結果,細胞内への陽性荷電したベクターや色素の移行が効率的にみられた.ミトコンドリアや葉緑体へのベクターの移行は直接証明は困難であった.2)BAC vector中にヒトミトコンドリアゲノムをクローニングして,増幅可能なベクターとする研究を行い,クローニングに成功した.しかし,このベクターは導入されたオルガネラ内で機能を発揮することが一部のみ確認できたにとどまった.3)葉緑体ゲノムはミトコンドリアゲノムに対して大きく,困難と考えられたので葉緑体ゲノムを一塊としてベクター化するのではなく,葉緑体ゲノムARF部をもつvectorの開発を行った.ベクターの機能的発現は現在検討中である.以上この研究にはベクターが巨大であることによる困難があり今後の研究課題である.

The purpose of this study is to determine whether there is an environmental DNA environment in which there are significant changes in new regulations. During the period of the study, the following results were obtained. 1) during the study period, the following results were obtained. 1) during the study period, the following results were obtained. 1) during the study period, the following results were obtained. 1) during the period of the study, the following results were obtained. 1) during the study period, the following results were obtained. 1) during the study period, the following results were obtained. The results showed that there was a significant difference in the migration rate of pigment in the cells. In the BAC vector, you may find that there is a problem in your research, and you may be successful. This is the first time that the machine has been able to make sure that it is necessary to make sure that it is possible to make sure that it is possible to do so. 3) in the wake of the accident, please do not know if it is necessary to make sure that the vector is in operation as soon as it is necessary to do so. The operation of the machine is now in the process of failure. The above research project is very difficult. The future research project is going to be very difficult.

项目成果

Expression of AIE-75 PDZ-domain protein induces G2/M cell cycle arrest in human colorectal adenocarcinoma SW480 cells

• DOI: 10.1016/j.canlet.2004.02.005
• 发表时间: 2004-08-10
• 期刊: CANCER LETTERS
• 影响因子: 9.7
• 作者: Hirai, A;Tada, M;Moriuchi, T
• 通讯作者: Moriuchi, T

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Prediction of lymph node metastasis and perineural invasion of biliary tract cancer by selected features from cDNA array data

• DOI: 10.1016/j.jss.2004.06.003
• 发表时间: 2004-12-01
• 期刊: JOURNAL OF SURGICAL RESEARCH
• 影响因子: 2.2
• 作者: Murakawa, K;Tada, M;Moriuchi, T
• 通讯作者: Moriuchi, T

Monti P, et al.: "Characterization of the p53 mutants ability to inhibit p73b transactivation using a yeast-based functional assay"Oncogene. 22. 5252-5260 (2003)

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