Integrated confocal luminescence spectrometer with spatiotemporal resolution and multiphoton excitation

功能材料的光物理研究对于深入了解其亚分子和超分子水平的发光激发态性质具有重要意义。对于地方、国家和国际层面正在进行和未来的项目，最先进的光谱和时间分辨发光分析技术需要与具有多光子激发能力的高空间和时间分辨率的共聚焦光学显微镜（包括发光寿命成像显微镜）相结合。这将构成一种独特的组合，目前还没有这种特殊形式。由于凝聚态相和界面的高度散射特性，在变温度下，用光谱、空间和时间分辨率测量溶液、均质基质、纤维、单层或多层、薄膜、非晶粉末、晶体、纳米或微粒中的单体或聚集分子发射体的发光是一项特别的挑战。这也适用于在培养基、细菌、生物膜、细胞器、细胞和组织中需要用光谱、空间和时间分辨率评估光敏剂、荧光或磷光探针的激发态特性。特别是，与基质、细菌和真核生物（宿主细胞或原生生物寄生虫）组分的特定相互作用需要就地监测。此外，双光子吸收截面将确定在均匀相。这些挑战将通过所要求的仪器来克服，这也将使双光子兴奋性的评估能够达到深层结构，用于生物成像和光疗。

The photophysical investigation of functional materials plays a fundamental role towards an in-depth understanding of luminescent excited state properties at sub- and supramolecular level. For ongoing and future projects at local, national and international level, state of the art spectrally and temporally resolved luminescence analysis techniques need to be combined with the high spatial and temporal resolution of confocal optical microscopy with multiphoton excitation capabilities (including luminescence lifetime imaging microscopy). This will constitute a unique combination that is presently not available in this particular form. Measuring the luminescence of monomeric or aggregated molecular emitters in solutions, homogeneous matrices, fibres, mono- or multilayers, thin films, amorphous powders, crystals, nano- or microparticles with spectral, spatial and temporal resolution at variable temperatures poses a particular challenge due to the highly scattering nature of condensed phases and interfaces. This also applies when the excited state properties of photosensitizers, fluorescent or phosphorescent probes need to be assessed with spectral, spatial and temporal resolution in culture media, bacteria, biofilms, organelles, cells and tissues. In particular, the specific interactions with matrixial, bacterial and eukaryotic (hosts cells or protozoic parasites) components need to be monitored in situ. Moreover, two-photon absorption cross-sections will be determined in homogeneous phases. These challenges will be overcome with the requested instrumentation, which will also enable the assessment of two-photon excitability to reach deep lying structures for bioimaging and phototherapy.