Integrated confocal luminescence spectrometer with spatiotemporal resolution and multiphoton excitation

功能材料的光物理研究对于在亚分子和超分子水平上深入理解发光激发态性质起着基础性作用。对于地方、国家和国际各级正在进行的和未来的项目，最先进的光谱和时间分辨发光分析技术需要与具有多光子激发能力的高空间和时间分辨率的共焦光学显微镜(包括发光寿命成像显微镜)相结合。这将构成一种独特的组合，这是目前这种特殊形式所不具备的。由于凝聚态和界面的高度散射特性，测量溶液、均质基质、纤维、单层或多层膜、薄膜、无定形粉末、晶体、纳米或微粒子中具有光谱、空间和时间分辨率的单体或聚集分子发射体在不同温度下的发光是一项特殊的挑战。这也适用于需要用光谱、空间和时间分辨率来评估光敏剂、荧光或磷光探针在培养介质、细菌、生物膜、细胞器、细胞和组织中的激发态性质时。特别是，需要现场监测与基质、细菌和真核生物(宿主细胞或原生动物寄生虫)成分的特定相互作用。此外，双光子吸收截面将在均相中确定。所要求的仪器将克服这些挑战，这也将使双光子兴奋性的评估能够到达生物成像和光疗的深层结构。

The photophysical investigation of functional materials plays a fundamental role towards an in-depth understanding of luminescent excited state properties at sub- and supramolecular level. For ongoing and future projects at local, national and international level, state of the art spectrally and temporally resolved luminescence analysis techniques need to be combined with the high spatial and temporal resolution of confocal optical microscopy with multiphoton excitation capabilities (including luminescence lifetime imaging microscopy). This will constitute a unique combination that is presently not available in this particular form. Measuring the luminescence of monomeric or aggregated molecular emitters in solutions, homogeneous matrices, fibres, mono- or multilayers, thin films, amorphous powders, crystals, nano- or microparticles with spectral, spatial and temporal resolution at variable temperatures poses a particular challenge due to the highly scattering nature of condensed phases and interfaces. This also applies when the excited state properties of photosensitizers, fluorescent or phosphorescent probes need to be assessed with spectral, spatial and temporal resolution in culture media, bacteria, biofilms, organelles, cells and tissues. In particular, the specific interactions with matrixial, bacterial and eukaryotic (hosts cells or protozoic parasites) components need to be monitored in situ. Moreover, two-photon absorption cross-sections will be determined in homogeneous phases. These challenges will be overcome with the requested instrumentation, which will also enable the assessment of two-photon excitability to reach deep lying structures for bioimaging and phototherapy.