Functional roles and regulation of HCN channels iin neuronal circuits

HCN通道在神经元回路中的功能作用和调节

• 批准号: 43597894
• 负责人: Professor Dr. Martin Biel
• 金额: --
• 依托单位: Department Pharmazie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/43597894?language=en
• 关键词: Functional; roles; regulation; HCN; channels

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are widely expressed in the CNS. While the electrophysiological properties of these channels have been investigated in quite detail in heterologous expression systems, the functional roles and the modulation of the channels in the context of neuronal circuits have been only poorly defined so far. Therefore, the first aim of this proposal is to identify and characterize regulatory proteins of HCN channels in different brain regions. Secondly, two genetic mouse models (HCN2/4cad) will be generated and analyzed to define the relevance of cAMP-dependent regulation of HCN channels in vivo. Finally, we will generate two mouse lines (HCN2-1/4 Switch mice) to study the correlation between the distinct biophysical properties of HCN channels and their functional roles in neuronal networks.

超极化激活环核苷酸门控（HCN）通道在中枢神经系统中广泛表达。虽然这些通道的电生理特性已经在异源表达系统中进行了相当详细的研究，但到目前为止，这些通道在神经元回路中的功能作用和调节还没有得到很好的定义。因此，本研究的第一个目的是鉴定和表征不同脑区HCN通道的调控蛋白。其次，将生成和分析两个遗传小鼠模型（HCN2/4cad），以确定camp依赖性HCN通道在体内的调控相关性。最后，我们将生成两个小鼠品系（HCN2-1/4开关小鼠）来研究HCN通道的不同生物物理特性与其在神经网络中的功能作用之间的相关性。