High Content Microscope (automated)
High Content Microscope (automated)
批准号:
436453180
负责人:
金额:
$0.0万
依托单位:
依托单位国家:
德国
项目类别:
Major Research Instrumentation
财政年份:
2020
资助国家:
德国
项目状态:
未结题
起止时间:
2019-12-31 至 --
中文摘要
具有高含量成像系统的表型单元是专门为自光合浮游生物及其相互作用物种(如轮虫、原生生物和病毒)以及异养原生生物的一系列表型性状的高通量测量而设计的。这为描述和机械地研究水生浮游生物和微生物群落之间的相互作用开辟了全新的可能性。具体来说,表型单元的设置允许测量数百个个体基因型的特征,并同时复制几个特征。所提出的表型单位对于我们对群落内生态和进化变化的机制理解是必不可少的,特别是对于检测性状变异和共变异随时间的变化,这需要同时记录单个细胞或单个动物的几个性状。表型单位的高通量允许在统计稳健的方式记录不同相互作用物种的特征。这样的表型单位在康斯坦斯大学是不可用的。
英文摘要
The phenotyping unit with a high-content imaging system is specifically designed for the high-through put measurement of a suit of phenotypic traits in auto-photosynthetic plankton organisms and their interacting species such as rotifers, protists and viruses, as well as heterotrophic protists. This opens up completely novel possibilities for describing and mechanistically investigating the interactions within aquatic plankton and microbial communities. Specifically, the set-up of the phenotyping unit allows measuring traits of hundreds of individual genotypes and replicates simultaneously and several traits. The proposed phenotyping unit is indispensable for our work on the mechanistic understanding of the ecological and evolutionary changes within communities, specifically for the detection of changes in trait variation and covariation over time, which requires the concurrent recording of several traits of individual cells or individual animals. The high throughput of phenotyping unit allows the characterization of traits of different interacting species be recorded in a statistically robust manner. Such a phenotyping unit is not available at the University of Constance.
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