Acquisition of an automated phase and fluorescence microscope for Cornell BRC Imaging Facility

项目总结/摘要 虽然康奈尔大学（伊萨卡校区）的NIH研究人员很幸运地获得了一些高端的 先进的显微镜（共聚焦，多光子，超分辨率，光片），他们缺乏一个主力倒置显微镜 其广泛地可用于需要低到中等放大率的实验。该提案描述了一项请求， Keyence紧凑型显微镜，具有相差和荧光功能，自动载物台和载物台顶部 孵化器该显微镜支持时间弹性成像、多位置成像、平铺和多孔板中的成像， 烧瓶。它将作为康奈尔大学生物技术资源中心（BRC）的一部分在成像设施内进行管理。数据 将利用已经建立的布拉迪斯拉发区域中心基础设施完成管理、调度和计费。显微镜 将提供给所有研究人员和校外，并将专门推进NIH资助的研究计划，重点是 对组织发育、免疫、癌症以及生物物理信号和通信的影响。

Project Summary/Abstract Though NIH researchers at Cornell University (Ithaca campus) are fortunate to have access to a handful of high-end advanced microscopies (confocal, multiphoton, super resolution, light sheet), they lack a workhorse inverted microscope that is broadly available for experiments requiring low to medium magnification. This proposal describes a request for a Keyence compact microscope with phase contrast and fluorescence capabilities, an automated stage and a stagetop incubator. The microscope supports timelaspe imaging, multi-position imaging, tiling, and imaging in multiwell plates and flasks. It will be managed within the Imaging Facility as part of Cornell’s Biotechnology Resource Center (BRC). Data management, scheduling and billing will be accomplished using already established BRC infrastructure. The microscope will be available to all researchers on and off campus, and will specifically advance NIH-funded research programs focused on tissue development, immunity, cancer, and biophysical signaling and communication.