Cloning and sequencing of a cDNA for poly(A)+RNA responsible for pole cell formation in Drosophila embryos.

对果蝇胚胎中负责极细胞形成的多聚 (A) RNA 的 cDNA 进行克隆和测序。

• 批准号: 59480018
• 负责人: OKADA Masukichi
• 金额: $ 3.71万
• 依托单位: Institute of Biological Sciences, University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59480018/
• 关键词: Drosophila; germ cell; pole cell; cytoplasmic determinant; gene cloning; cDNA; rRNA; rRNA; ミトコンドリア; 生殖系列; CDNA; ミトコンドリアrRNA

Final goal of the present project is to elecidate the molecular basis for the function of polar plasm as the germ plasm. In Drosophila, polar plasm is known to autonomously initiate pole cell formation and commit the pole cells to develop to germ cells. We already showed in Drosophila that a maternally transcribed poly(A)^+RNA was responsible for pole cell formation, adn proposed that this RNA was a component of germ plasm. In this project we cloned cDNA of this poly(A)^+RNA, and determined the nucleotide sequence of the cDNA. A search in a DNA data base by a computer program showed that this cDNA sequence is strictly homologous with mitochondrial large ribosomal DNA but not with nuclear DNA.

本课题的最终目的是揭示极性质作为种质的分子基础。在果蝇中，极细胞质可以自主启动极细胞的形成，并使极细胞发育为生殖细胞。我们已经在果蝇中证明，母体转录的poly（A）^+RNA负责极细胞的形成，并提出这种RNA是生殖质的一种成分。在本项目中，我们克隆了该poly（A）^+RNA的cDNA，并测定了该cDNA的核苷酸序列。用计算机程序检索DNA数据库表明，该cDNA序列与线粒体大核糖体DNA严格同源，但与核DNA不同源。

项目成果

M.Okada & S.Kobayashi: Development. Growth & Differentiation. 29. 185-192 (1987)

冈田先生

E. Maruo & M. Okada: "Monoclonal antibodies against Drosophila ovarian antigens. An approach to the analysis of maternal factors regulating early development." Cell Differentiation. 20. 45-54 (1987)

E·丸尾

F.Maruo & M.Okada: Cell Differentiation. 20. 45-54 (1987)

丸尾

S. Togashi, S. Kobayashi & M. Okada: "Function of maternal mRNA as a cytoplasmic factor responsible for pole cell formation in Drosophila embryos." Developmental Biology. 118. 352-360 (1986)

富坚 S.、小林 S.

M.Okada: Zoological Science. 3. 573-583 (1986)

M.Okada：动物学。