Proliferation and differention of oogonia in Drosophila oogenesis

在许多动物中，配子发生发生在生殖系包囊中。果蝇卵母细胞在含有16个卵原细胞的囊中发育，这些卵原细胞通过细胞间桥相互连接。胞质结构，多聚体，延伸通过包囊内的每个细胞间桥。为了深入了解多聚体在控制包囊形成和每个包囊内细胞质不对称性中的作用，我们使用单抗对果蝇多聚体的一个组分进行了分子分析。本研究获得的新发现如下。结论：1.多聚体含有细胞骨架蛋白。在早期卵巢囊肿中，四体成分的分布不均匀。这是在胞囊中观察到的最早的分子不对称。雄性生殖系细胞和雌性生殖系细胞中均存在多聚体。观察到雄性和雌性多融合体间的形态差异。这些差异受种系性别决定基因控制。男性和女性生殖系干细胞中也含有四倍体成分。通过细胞内融合体的不对称分布，可以识别干细胞内的细胞极性。

In many animals gametogenesis takes place in germline cysts. Drosophila oocytes develop within cysts containing 16 oogonial cells that are interconnected by intercellular bridges. A cytoplasmic structure, polyfusome, extends through each intercellular bridge in the cyst. To gain insight into the role of polyfusome in controlling the cyst formation and the cytoplasmic asymmetry within each cyst, we have carried molecular analyzes on a component of Drosophila polyfusome using a monoclonal antibody. New findings obtained in this study are as follows.1. Polyfusome contained cytoskeletal proteins.2. The unegual distribution of the fusomal component was found in the early ovarian cysts. This is the earliest molecular asymmetry observed in the cyst.3. Polyfusome was found in the male germline cells as well as in the female germline cells.4. Morphological differences between male and female polyfusomes were observed. These differences were under the control of the germline sex determination genes.5. The fusomal component was also contained in male and female germline stem cells. The cellular polarity in stem cell could be recognized by the asymmetrical distribution of the fusome in the cell.