Proliferation and differention of oogonia in Drosophila oogenesis

果蝇卵子发生中卵原细胞的增殖和分化

• 批准号: 05680632
• 负责人: OKADA Masukichi
• 金额: $ 1.22万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05680632/
• 关键词: Drosophila; Oogenesis; Spermatogenesis; Oogonium; Spermatogonium; Cytoplasmic Bridge; Polyfusome; Stem Cell; 不完全分裂

In many animals gametogenesis takes place in germline cysts. Drosophila oocytes develop within cysts containing 16 oogonial cells that are interconnected by intercellular bridges. A cytoplasmic structure, polyfusome, extends through each intercellular bridge in the cyst. To gain insight into the role of polyfusome in controlling the cyst formation and the cytoplasmic asymmetry within each cyst, we have carried molecular analyzes on a component of Drosophila polyfusome using a monoclonal antibody. New findings obtained in this study are as follows.1. Polyfusome contained cytoskeletal proteins.2. The unegual distribution of the fusomal component was found in the early ovarian cysts. This is the earliest molecular asymmetry observed in the cyst.3. Polyfusome was found in the male germline cells as well as in the female germline cells.4. Morphological differences between male and female polyfusomes were observed. These differences were under the control of the germline sex determination genes.5. The fusomal component was also contained in male and female germline stem cells. The cellular polarity in stem cell could be recognized by the asymmetrical distribution of the fusome in the cell.

在许多动物中，配子发生发生在生殖细胞包囊中。果蝇卵母细胞在含有16个卵原细胞的包囊内发育，这些卵原细胞通过细胞间桥相互连接。胞质结构，多丝体，延伸通过每个细胞间的桥梁在囊肿。为了深入了解多丝体在控制包囊形成和每个包囊内的细胞质不对称性中的作用，我们使用单克隆抗体对果蝇多丝体的一个组分进行了分子分析。本研究的新发现如下：1.多丝体含有细胞骨架蛋白.在早期卵巢囊肿中发现梭体成分的不均匀分布。这是在囊肿中观察到的最早的分子不对称性。在雄性生殖细胞和雌性生殖细胞中都发现了多丝体.雄性和雌性多丝体之间的形态差异进行了观察。这些差异受生殖系性别决定基因的控制.梭体成分也包含在男性和女性生殖系干细胞中。通过融合体在细胞内的不对称分布可以识别干细胞的细胞极性。

项目成果

Okada, M., Amikura, R., Kitamura, T., Endo, K.and Kobayashi, S.: "Molecular analysis of germ line formation in Drosophila embryos." Proc.Arthropod.Embryol.Soc.Jpn.29. 1-6 (1994)

Okada, M.、Amikura, R.、Kitamura, T.、Endo, K. 和 Kobayashi, S.：“果蝇胚胎种系形成的分子分析”。

I.Sugimura: "On the allelism of fs(1)K1274 and Sex-lethal mutations" Drosophila Information Service. 75. 147-148 (1994)

I.Sugimura：“关于 fs(1)K1274 的等位性和性致死突变”果蝇信息服务。

Kitamura, T.: "Developmentally regulated splicing of the third intron of P element in somatic tissues in Drosophila embryos." Develop. Growth & Differ. 35. 67-73 (1993)

Kitamura, T.：“果蝇胚胎体组织中 P 元件第三个内含子的发育调控剪接。”

Okada, M.: "Molecular analysis of germ line formation in Drosophila embryos." Proc. Arthropod. Embryol. Soc. Jpn.29. 1-6 (1994)

Okada, M.：“果蝇胚胎种系形成的分子分析。”

Asaoka, M.: "Digitonin activates different sets of puff locidepending oh developmental stages in Drosophila melanogastertsalivary glands." Develop. Growth & Differ. 36. 605-614 (1994)

Asaoka, M.：“洋地黄皂苷根据果蝇唾液腺的发育阶段激活不同的泡芙基因座。”