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Study on the central mechanism for gonadotropin secretion in the female rat by brain transplantation technique.

脑移植技术研究雌性大鼠促性腺激素分泌的中枢机制。

基本信息

批准号：
59480114
负责人：
TANAKA Fukuko
金额：
$ 2.94万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

项目摘要

1. Method for transplantation of catecholamine-neuron in the brain: The neural tissue including A-6 group noradrenaline (NA)-neurons in the locus coeruleus (for NA-rich transplant) or A-10 group doapmine (DA)-neurons in the substantia nigra (for DA-rich transplant) was punched out of the coronal slice dissected from the medulla or midbrain of newborn rats, respectively, with a metal cannula and was stereotaxically positioned in the third ventricle at the level of the preoptic region of cycling rats. Surviving transplant was confirmed to have catecholaine neurons in the Falck-Hillarp fluorescence method.2. Reproductive function of the female rats with catecholamine-neuron transplants in the third ventricle: The female rat that had surviving NA-neuron rich transplant frequently showed prolongation of estrus, indicating that ovulation was severely impaired. However, after ovariectomy, they showed a pulsatile secretion of LH with a remarkably large amplitude. The DA-neuron rich transplants … More sustained the estrous cycle unchanged, and did not change pulsatile secretion of LH after ovariectomy, too. The results suggested that the NA-neurons rich transplants at the preoptic region somehow inhibited gonadotropin secretion in the intact rat, whereas they facilitated it in ovariectomized rats.3. Blood pressure of the rats with catecholamine-neuron transplant in the third ventricle: The rats with either transplant, NA-neurone ro DA-neuron, showed a long lasting hypotension. The hypotensive effect was exerted even on the hypertension produced by salt-loading.4. Effect of estrogen-progesterone treatment on the gonadotropin secretion in the gonadectomized male rat with the ventricular implantation of the preoptic suprachiasmatic nucleus from the female newborn: The preoptic suprachiasmatic nucleus has been considered as the key locus for positive feedback action of progesterone on gonadotropin secretion in the female rat. Transplantation of such a neural tissue in the male brain was not effective to induce LH surge, while FSH secretion was induced. Less

1. 脑内儿茶酚胺神经元移植方法：用金属套管分别从新生大鼠髓质或中脑解剖的冠状切片中取出新生大鼠蓝斑处a -6组去甲肾上腺素（NA）神经元（富NA移植）或黑质处a -10组多巴胺（DA）神经元（富DA移植）的神经组织，立体定位于循环大鼠视前区水平的第三脑室。Falck-Hillarp荧光法证实存活的移植细胞有儿茶酚碱神经元。第三脑室儿茶酚胺神经元移植雌性大鼠生殖功能：富na神经元移植存活的雌性大鼠频繁出现发情期延长，表明排卵受到严重损害。然而，卵巢切除术后，他们表现出脉动性的LH分泌，幅度非常大。富含da神经元的移植更能维持卵巢的动情周期不变，也未改变卵巢切除术后的脉动性LH分泌。结果表明，在完整大鼠视前区植入富含na神经元的移植细胞以某种方式抑制了促性腺激素的分泌，而在去卵巢大鼠则促进了促性腺激素的分泌。第三脑室儿茶酚胺-神经元移植大鼠血压：na -神经元和da -神经元移植大鼠均出现长期低血压。甚至对盐负荷引起的高血压也有降压作用。雌激素-孕酮处理对雌性新生儿视交叉上核脑室植入的去性腺化雄性大鼠促性腺激素分泌的影响：视交叉上核被认为是孕激素对雌性大鼠促性腺激素分泌正反馈作用的关键位点。将该神经组织移植到男性脑内不能有效诱导LH激增，但可诱导FSH分泌。少