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Sex differences in the stress responses in the amygdala

杏仁核应激反应的性别差异

基本信息

批准号：
14570061
负责人：
TANAKA Fukuko
金额：
$ 2.62万
依托单位：
Yokohama City University Graduate School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570061/
关键词：
Sex difference Acetylcholine Dopamine Serotonin Stress Housing Amygdala Hippocampus

项目摘要

To examine the effect of the restriction of environmental space on the stress response of the hippocampal acetylcholine (ACh) release, an in vivo microdialysis study was performed in male and female rats after 4-day housing in a large cylindrical cage (diameter = 35 cm) or a small cylindrical cage (diameter =19 cm). Dialysates and blood samples were taken from 1100 h to 1600 h in freely moving rats, but restraint stress was applied from 1200 h to 1300 h. Male rats living in a large cage showed greater stress response of the A Ch release than female rats living in a large cage. In addition, the stress response of ACh release in the hippocampus was attenuated in male rats living in a small cage, while female rats showed a significant increase in the ACh release in female rats living in a small cage. We surmise that there may be a sex difference in the effect of housing condition in the stress response of ACh release in the hippocampus.To examine the sex difference in the stress response of serotonin (5HT) and dopamine (DA) release in the basolateral amygdala (BLA), an in vivo microdialysis study was performed in intact male and cycling female (diestrus 2) rats. Basal 5HT release in the BLA was low without sex difference. Although the 5HT release promptly increased with the onset of the restraint stress in both sexes, the magnitude of the stress response in male rats was significantly less than in female rats. In contrast, male rats showed higher basal DA release in the BLA than female rats. Although the DA release promptly increased with the onset of the restraint stress in female rats, male rats showed no significant stress response. These results indicate that sex and/or gonadal steroid hormone environment can affect the stress response of 5HT and DA release in the BLA in rats.

为探讨环境空间限制对大鼠海马乙酰胆碱(ACh)释放应激反应的影响，在大圆筒笼子(直径35 cm)和小圆筒笼子(直径19 cm)中对出生4天的雄性和雌性大鼠进行了体内微透析研究。自由活动大鼠在1100h~1600h采集透析液和血样，而在1200h~1300h施加束缚应激，大笼子里的雄性大鼠比大笼子里的雌性大鼠表现出更大的A-CH释放应激反应。此外，生活在小笼中的雄性大鼠海马区ACh释放的应激反应减弱，而雌性大鼠在小笼中生活的雌性大鼠的ACh释放显著增加。我们推测，居住条件对海马区ACh释放应激反应的影响可能存在性别差异。为了研究杏仁基底外侧核(BLA)5-羟色胺(5HT)和多巴胺(DA)释放应激反应的性别差异，对正常雄性和动情间期雌性大鼠进行了体内微透析研究。BLA基础5-羟色胺释放水平较低，无性别差异。尽管随着束缚应激的开始，雌雄大鼠的5-羟色胺释放迅速增加，但雄性大鼠的应激反应幅度明显小于雌性大鼠。相比之下，雄性大鼠在BLA中的基础DA释放量高于雌性大鼠。虽然雌性大鼠的DA释放量随着束缚应激的开始而迅速增加，但雄性大鼠没有表现出明显的应激反应。这些结果表明，性别和/或性腺类固醇激素环境可影响大鼠血中5-羟色胺的应激反应和多巴胺的释放。