Surface Chemical Study on Polymer Latex for Immuno-diagonosis

免疫诊断用聚合物乳胶的表面化学研究

• 批准号: 60470081
• 负责人: SUZAWA TOSHIRO
• 金额: $ 3.26万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60470081/
• 关键词: Immuno-diagnosis; Dispersion-stability; 表面特性; 凝集性; 分散安定性

Copolymer latices having functional and hydrophilic groups on their surfaces, i. e., styrene/2-hydroxyethyl methacrylate (HEMA)/acrylic acid (AA) copolymer [P(St/HEMA/AA)] and styrene/acrylamide (AAm)/AA copolymer [P(St/AAm/AA)] latices expected for immuno-diagnosis were prepared without emulsifier. Electron micrographs of these latices revealed that the latex particles were highly monodisperse. Viscosity and <zeta> -potential measurements of latex dispersions showed that hydrogel layers derived from poly-HEMA ( or poly-AAm) and poly-AA existed on the surfaces of these latices.The stability of latex dispersions increased with increasing the quantity of HEMA or AAm used in the copolymerization, and changed by pH. Further, the coagulation rate of latex dispersions increased with an increase of temperature. This is probably because the steric repulsion between latex particles decreases with increasing temperature.Furthermore, the coagulation rate of BSA-covered latex particles increased in the lower concentration region of NaCl, but decreased in the higher NaCl concentration region. Nevertheless in general, it was found that the stability of latex dispersions was enhanced by covering with BSA molecule.

在其表面上具有官能基团和亲水基团的共聚物胶乳，即。例如，在无乳化剂条件下制备了苯乙烯/甲基丙烯酸羟乙酯（HEMA）/丙烯酸（AA）共聚物[P（St/HEMA/AA）]和苯乙烯/丙烯酰胺（AAm）/AA共聚物[P（St/AAm/AA）]胶乳。这些胶乳的电子显微照片显示，胶乳颗粒是高度单分散的。乳液的粘度和<zeta>电位测定表明，乳液表面存在由聚HEMA（或聚AAm）和聚AA形成的水凝胶层，乳液的稳定性随共聚单体用量的增加而增加，随pH值的变化而变化，乳液的凝聚速率随温度的升高而增加。这可能是因为随着温度的升高，乳胶粒之间的空间排斥力减小，而且BSA包覆乳胶粒的凝聚速率在低浓度NaCl区域增大，而在高浓度NaCl区域减小。尽管如此，一般情况下，它被发现，胶乳分散体的稳定性增强与BSA分子的覆盖。

项目成果

H.Tamai;H.Shirahama;T.Ekimoto;T.Suzawa: 油化学. 35. 737-741 (1986)

H. Tamai；H. Shirahama；T. Suzawa：石油化学。

須澤利郎著,筏義人編: "高分子表面の基礎と応用、上巻" 化学同人, 24 (1986)

须泽敏郎着，嘉藤义人编：《高分子表面的基础与应用》第1卷》化学同人，24（1986）

H.Tamai;Y.Ishii;Y.Kodera;T.Suzawa: J.Colloid Interface Sci.114. 267-269 (1986)

H.Tamai；Y.Ishii；Y.Kodera；T.Suzawa：J.胶体界面科学.114。

H.Tamai;T.Murakami;T.Suzawa: J.Applied Polymer Sci.30. 3857-3866 (1985)

H.Tamai；T.Murakami；T.Suzawa：J.Applied Polymer Sci.30。

K.Kawasaki;H.Tamai;T.Suzawa: Textile Res.J.56. 598-603 (1986)

K.Kawasaki；H.Tamai；T.Suzawa：纺织研究 J.56。