Mechanism and epidemiological significance of high contaminations of Vibrio parahaemolyticus to estuarine molluscs

副溶血性弧菌对河口软体动物高污染的机制及流行病学意义

• 批准号: 60480094
• 负责人: KUMAZAWA Norichika
• 金额: $ 4.16万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480094/
• 关键词: Vibrio parahaemolyticus; Clithon retropictus; Corbicula japonica; colonization; enzyme-linked immunoassay; hemocytes; 神奈川溶血毒; 汽水産貝類

From our evidences that high levels of Vibrio parahaemolyticus were detected from two molluscs, Clithon retropictus and Corbicula japonica, at Hashizu estuary and that many strains detected from C. retropictus were estimated as Kanagawa-positive in immunodiffusion, close relationships between V. parahaemolyticus and estuarine molluscs were suspected. Thus, survivals of V. parahaemolyticus D-3 (Kanagawa-positive) and R-13(Kanagawa-negative) and Escherichia coli YS-2 in these molluscs held in aquaria were investigated. These strains survived in C. retropictus for 21 days. Strains D-3 and R-13, but not YS-2, survived in C. japonica for 14 days. These strains were eliminated from Nerita albicilla, a marine mollusc, within 72 hours.Method to detect Kanagawa hemolysin from culture-supernatants of V. Parahaemolyticus strains utilizing enzyme-linked immunoassay was developed. inhinitor(s) in the supernatants was inactivated by boiling for 30 min. Among boiled-supernatants of estuarine strains, the hemolysin was detected from 9 strains in 36 strains derived from C. retropictus and from 21 in 35 derived from C. japonica, whose titers were between 40 and 80 units/ml compared with 1280 units/ml in clinical isolates.As successful colonizations of V. parahaemolyticus strains in the estuarine molluscs was supposed to be derived from defective responses of the molluscan hemocytes, chemotactic responses of the hemocytes to strains D-3 R-13 and YS-2 were measured in blind well chambers. Hemocytes of N. albicilla showed an active migration to all strains while those of C. retropictus showed no migration to any organisms. Of C. japonica hemocytes, adhesive cells showed no migration to any organisms while non-adhesive cells migrated only to YS-2.

从桥津河口两种贝类中检出高水平的副溶血性弧菌，以及从日本蚬中检出的多株副溶血性弧菌。retropictus在免疫扩散中被估计为神奈川阳性，副溶血性弧菌与河口软体动物之间的密切关系被怀疑。因此，调查了副溶血性弧菌D-3（神奈川阳性）和R-13（神奈川阴性）以及大肠杆菌YS-2在水族箱中的存活情况。这些菌株在C. retropictus 21天。D-3和R-13在C.粳稻14天。建立了用酶联免疫法检测副溶血弧菌培养上清中神奈川溶血素的方法。在36株C. retropictus和21/35的C.由于副溶血性弧菌在河口软体动物体内的成功定殖可能是由于软体动物血细胞的反应缺陷所致，因此，在盲孔培养箱中测定了D-3、R-13和YS-2两株副溶血性弧菌血细胞的趋化反应。血细胞N.白色念珠菌对所有菌株均表现出活跃的迁移，而白色念珠菌对所有菌株均表现出活跃的迁移。retropictus没有向任何生物体迁移。梭在日本血细胞中，粘附细胞未显示出向任何生物体迁移，而非粘附细胞仅向YS-2迁移。

项目成果

Norichika H. Kumazawa: "Preliminary analyses on persistence of Vibrio parahaemolyticus in a brackish water clam, Corbicula japonica." The Japanese Journal of Veterinary Science. 48. 267-271 (1986)

Norichika H. Kumazawa：“对咸水蛤蚬中副溶血弧菌持久性的初步分析。”

Norichika H. KUMAZAWA: The Japanese Journal of Veterinary Sciene.

Norichika H. KUMAZAWA：日本兽医科学杂志。

Norichika H. KUMAZAWA: The Jaoanese Journal of Veterinary Sciense. 48. 267-271 (1986)

Norichika H. KUMAZAWA：《日本兽医科学杂志》。

Norichika H. Kumazawa: "Survival of Vibrio parahaemolyticus in two gastropod molluscs, Clithon retropictus and Nerita albicilla." The Japanese Journal of Veterinary Science.

Norichika H. Kumazawa：“副溶血性弧菌在两种腹足类软体动物 Clithon retropictus 和 Nerita albicilla 中的存活。”

Norichika H.Kumazawa: The Japanese Joural of Veterinary Science. 48. 267-271 (1986)

Norichika H.Kumazawa：日本兽医科学杂志。