Development of arteriosclerosis marker for health promotion and detection

本项目调查了几种血清和尿胶原代谢标志物作为动脉硬化指标的有效性，因为动脉硬化涉及血管壁的变化，即&lt；III&gt；型胶原和基底膜成分的积累。因此，我们研究了血清Ⅲ型前胶原肽、血清层粘连蛋白、尿羟脯氨酸排泄量与动脉硬化的关系。对121例健康体检者(男84例，女37例)和28例无糖尿病并发症的男性患者进行了研究。血清总胆固醇、甘油三酯与眼底及心电图的动脉硬化改变无相关性，不宜作为动脉硬化指标。老年男性尿羟脯氨酸排泄量和血清&lt；III&gt；型前胶原肽水平略高，但与动脉硬化改变无关。另一方面，老年男性和女性的血清层粘连蛋白水平较高。然而，动脉硬化组的血清层粘连蛋白水平较非动脉硬化组有升高的趋势，但无统计学意义。血清层粘连蛋白水平与年龄(r=0.231，p0.05)、血清总胆固醇(r=0.330，p0.01)、甘油三酯(r=0.241，p0.05)呈显著正相关。这些结果提示，血清层粘连蛋白水平可作为动脉硬化的有用指标，因为它可能反映了基底膜生物合成的增加。

The present project has investigated the usefulness of several serum and urine markers for collagen metabolism as an indicator for arteriosclerosis, because arteriosclerosis involves the alterations of blood vessels wall, that is, the accumulation of type <III> collagen and basement membrane components. Therefore, serum type <III> procollagen peptide as an indicator for biosynthesis of type <III> collagen, serum laminin as an indicator for biosynthesis of basement membrane glycoprotein, and urinary hydroxy proline excretion were investegated in relation with arteriosclerosis. 121 health-checked subjects (84 males and 37 females) and 28 male patients with diabetes mellitus without any diabetic complications were studied. Serum total cholesterol and triglyceride were not correlated with their arteriosclerotic changes on optic fundi and electrocardiogram and proved to be inappropriate for arteriosclerotic indicator. Urinary hydroxyproline excretion and serum levels of type <III> procollagen peptide were slightly higher among older-aged males but not correlated with arteriosclerotic changes. On the other hand, serum laminin values were higher among older aged males and females. Nonetheless, there was a higher tendency of the serum laminin values among arteriosclerotic cases compared to those of non-arteriosclerotic cases without statistical significance. Serum laminin values showed significant positive correlations with age (r=0.231, p 0.05), serum total cholesterol (r=0.330, p 0.01) and triglyceride (r=0.241, p 0.05). These findings suggested that the serum laminin value can be useful indicator for arteriosclerosis, because it may reflect increased biosynthesis of basement membrane.