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Studies on the Role of Peripheral Mononuclear Cell derived Fibroblast Proliferating Factor in Hepatic Fibrosis of CAH Patients.

外周单核细胞源性成纤维细胞增殖因子在CAH患者肝纤维化中作用的研究。

基本信息

批准号：
60480213
负责人：
ICHIRO URUSHIZAKI
金额：
$ 0.64万
依托单位：
Sapporo Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480213/
关键词：
Hepatic Fibrosis Fibroblast Proliferating Factor インターロイキン1

项目摘要

In order to clarify the immunological mechanism of hepatic fibrosis, peripheral mononuclear cells (PMNC) from patients with chronic hepatitis and liver cirrhosis were examined to know whether they release some fibroblast proliferating factors in response to PHA stimulation or not.The results obtained were as follows:1) In the culture medium of PHA stimulated PMNC from patients with chronic hepatitis and liver cirrhosis, significantly higher activities of fibroblast proliferation were demonstrated as compared to those from normal subjects.2) The cultured medium obtained from CAH was fractionated on high performance liquid chromatography and the activities were recovered in two peaks with a molecular weight of 60,000 (FPF- <I> ) and 20,000 daltons (FPF- <II> ), respectively. Both active peaks coincide with those of collagen synthesis.3) The flat bed isoelectric focusing study of FPF- <I> and FPF- <II> revealed that p. <I> . of both fractions existed at 5:1.4) FPF- <I> and <II> coincide with active peaks of interleukin 1 (IL-1) activity as assessed by thymocyte proliferating assay and CTLL proliferating assay.5) The macrophage fractions of PMNC from patients with chronic hepatitis and liver cirrhosis were found to secrete a fibroblast proliferating factor in relatively higher concentrations by the stimulation of LPS.It was assumed from the above results that the macrophage population in PMNC of chronic hepatitis or liver cirrhosis produces more IL-1 than normal subjects and thus the secreted IL-1 caused proliferation of fibroblast in the liver.

为了阐明肝纤维化的免疫学机制，对慢性肝炎和肝硬变患者外周血单个核细胞(PMNC)进行了检测，以了解PHA刺激PMNC是否释放某些成纤维细胞增殖因子。结果如下：1)在PHA刺激的慢性肝炎和肝硬变患者PMNC的培养上清液中，成纤维细胞的增殖活性明显高于正常人。Ii和gt；)。这两个活性峰与胶原合成物的活性峰一致。3)平板层等电聚焦研究显示，p。两组分的比例为5：1.4)，两者与胸腺细胞增殖试验和CTLL增殖试验测定的IL-1活性峰值一致。5)慢性肝炎和肝硬变患者PMNC的巨噬细胞组分在LPS的刺激下能分泌相对较高浓度的成纤维细胞增殖因子。