Optogenetic membrane manipulation of protein targeting to mitochondria and plastids (A13)

我们的数据表明，一些蛋白质靶向的质体在有氧条件下在衣原体单胞菌reinhardtii，双靶向的线粒体和质体在厌氧条件下（和损失的线粒体Δ psi）。我们计划产生光遗传学可控的细胞器，无论是在膜整合的视蛋白来操纵Δ psi，以及通过光遗传学开关调节膜结合的受体平台和磷酸酶。我们的工作将（i）揭示靶向序列和Δ psi -和受体介导的膜身份的相互作用，确保正确的靶向，和（ii）实现新的光遗传学工具，用于理解和控制C细胞过程。莱因哈德氏菌

Our data demonstrate that some proteins targeted to the plastid under aerobic conditions in Chlamydo-monas reinhardtii, are dual-targeted to mitochondrion and plastid under anaerobic conditions (and loss of mitochondrial delta psi). We plan to generate optogenetically controllable organelles, both in terms of membrane-integrated opsins to manipulate delta psi, as well as modulating membrane-bound receptor platforms and phosphatases through optogenetic switches. Our work will (i) shed new light on the interplay of targeting sequences and delta psi - and receptor-mediated membrane identity that secures correct targeting, and (ii) implement new optogenetic tools for understanding and controlling cellular processes in C. reinhardtii.