Optogenetic membrane manipulation of protein targeting to mitochondria and plastids (A13)

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Our data demonstrate that some proteins targeted to the plastid under aerobic conditions in Chlamydo-monas reinhardtii, are dual-targeted to mitochondrion and plastid under anaerobic conditions (and loss of mitochondrial delta psi). We plan to generate optogenetically controllable organelles, both in terms of membrane-integrated opsins to manipulate delta psi, as well as modulating membrane-bound receptor platforms and phosphatases through optogenetic switches. Our work will (i) shed new light on the interplay of targeting sequences and delta psi - and receptor-mediated membrane identity that secures correct targeting, and (ii) implement new optogenetic tools for understanding and controlling cellular processes in C. reinhardtii.