权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Basic study on brain-cell transplantation from a clinical point of view

从临床角度进行脑细胞移植的基础研究

基本信息

批准号：
60480333
负责人：
TSUBOKAWA Takashi
金额：
$ 3.84万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480333/
关键词：
Brain-cell transplantation Brain edema Raphe nucleus Serotonin 海馬

项目摘要

The present study demonstrated, by means of immunohistochemical, neurochemical, neurophysiological as well as behavioral analysis, that fetal raphe cells transplanted into the hippocampus with a cell-suspension method can survive, give off fiber-outgrowth and release 5-HT in the adult host brain, and restore host brain functions. It was suggested that transplanted raphe cells may be regarded as an intrahippocampal raphe nucleus from a view of their efferent function. However, it was also demonstrated that these raphe cells were not under normal afferent controls. Further studies are necessary to answer whether normal afferent controls develop over a prolonged period of time. The present study also demonstrated that fetal raphe cells transplanted into the host brain associated with hydrocephalic interstitial edema can survive and give off fiber-outgrowth in spite of the presence of edema itself and increased intracranial pressure. Furthermore, the results indicated that, as far as hydrocephalic interstitial edema is concerned, the edema per se may not only be just susceptible of transplantation but provide more useful environment for survival of transplanted cells. The transplantation method would be useful as an in vivo culture system to evaluate the role of differential environments associated with edema of diverse etiology on cell survivability and fiber-outgrowth.

本研究通过免疫组织化学、神经化学、神经生理学及行为学分析等方法，证明细胞悬浮法将胎儿中缝细胞移植到海马区后，可在成年宿主脑内存活、纤维生长和释放5-羟色胺，恢复宿主脑功能。从传出功能看，移植的中缝细胞可视为海马区的中缝核团。然而，也证明了这些中缝细胞不在正常的传入对照下。需要进一步的研究来回答正常的传入控制是否会在较长一段时间内发展起来。本研究还表明，移植到宿主脑内的胎儿中缝细胞与脑积水间质水肿相关，尽管存在水肿和颅内压升高，但仍能存活并发出纤维生长。此外，结果表明，就脑积水间质水肿而言，这种水肿本身可能不仅是移植的易感因素，而且为移植细胞的生存提供了更有利的环境。该移植方法可作为体内培养系统，用于评估与不同病因的水肿相关的不同环境对细胞存活和纤维生长的影响。