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Experimental study of the embryology in the ear

耳胚胎学的实验研究

基本信息

批准号：
60480380
负责人：
MASUDA YU
金额：
$ 2.37万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

1. Normal development of the ear The normal development of the external and middle ear in the mouse was observed histologically. Especially, main structures in the middle ear were clarified on the embryological stapes. The developmental origin of the stapes footplate was investigated and confirmed to be derived from the otic capsule in the vestibular surface of the footplate. On the external ear, formation of the tympanic membrane was observed at the 10 or 11 days after the birth.2. The experimental studies of teratogenicity of Vitamin A and the inhibitory effect of alkaloid on it.Mother rats were divided into 4 groups consisted of rats treated with Vitamin A, Vitamin A and Cepharanthin ( a kind of alkaloid), Cepharanthin and nothing as control at day 10 of pregnancy. In the results, the frequency of external malformations in fetuses induced by maternal hypervitaminosis A was remarkably decreased in the group treated with Cepharanthin. Further more, the same meterials were stained with … More bone and cartilage staining method and thefindings of their middle ear region were observed. From these studies, the inhibitory effect of Cepharanthin to the teratogenic activity of excessive Vitamin A on the external and internal malformations was suggested concerning with the membrane stabilizing effect of Cepharanthin.3. Embryological observations of the mouse ear using organ culture techniqueThe ear region in the 10th gestation day mouse embryo was explanted into the organ culture system and was cultured for 4 days to 10 days.The tissues of ear region cultured for 4 or 5 days showed the developing sensory epithelium in the inner ear, cartilaginous otic capsule, acoustico-facial ganglion cells, facial nerve fibers and anlage of ossicles in their histological specimens.4. Developmental study of the rat ear using whole embryo culture technique.Rat embryos were explanted at 11.5 days of gestation and cultured for 24 hours using the whole embryo culture technique.Morphological and histological examinations were made on the ear region of the cultured and control embryos. In the control group, otocysts were simple and oval in shape.In the cultured embryos, otocysts elongated and narrowed and endolymphatic ducts could clearly be seen. Less

1.耳的正常发育组织学观察小鼠外耳和中耳的正常发育。特别是在胚胎性中耳骨上清楚地显示了中耳的主要结构。研究了距骨底板的发育起源，并证实其起源于前庭表面的耳囊。外耳在出生后10天或11天可见鼓膜形成。维生素A致畸及生物碱抑制作用的实验研究。将孕鼠分为4组，每组10只，每组10只，每组10只，分别给予维生素A、维生素A和三尖杉酯碱(一种生物碱)、三七总碱和空白对照。结果表明，治疗组可显著降低母体维生素A过多所致胎儿外畸形的发生率。此外，还对相同材料进行了…染色观察更多的骨和软骨染色方法及中耳区的表现。通过这些研究，提出了天麻素对过量维生素A致畸胎活性的抑制作用可能与脑红素的膜稳定作用有关。用器官培养技术对小鼠耳的胚胎学观察将孕10天的小鼠胚胎的耳区移植到器官培养系统中，培养4天至10天，培养4天或5天的耳区组织在组织学标本中可见发育中的内耳感觉上皮、软骨囊、听面神经节细胞、面神经纤维和听小骨突起。采用全胚胎培养技术对大鼠耳部发育进行研究。于妊娠11.5天取大鼠胚胎，用全胚胎培养技术培养24小时，对培养的胚胎和对照胚胎的耳部进行形态和组织学检查。对照组耳囊呈单纯型、椭圆形，培养的胚胎可见耳囊变长变窄，内淋巴管清晰可见。较少