ANALYSIS OF THE ROLE OF PUTATIVE CHEMICAL MEDIATORS IN INFLAMMATION AND ALLERGY USING THE AIR POUCH MODEL OF INFLAMMATION.

1. 项目目的：通过本实验室开发的各种炎症气囊模型，试图研究各种假定的炎症和过敏化学介质的作用。结果与结论：(1)卡拉胶诱导炎症：炎症部位迟缓激肽水平与早期1.5hr血浆渗出强度密切相关。缓激肽抑制剂治疗可降低缓激肽水平，同时降低兴奋程度。这些结果清楚地表明缓激肽在卡拉胶诱导炎症的早期阶段有明确的作用。(2)高岭土诱导炎症：证实注入气囊的道林悬浮液通过激活缓激肽-激肽系统产生缓激肽而引起血浆渗出。(3)变应性炎症：变应性炎症模型眼袋液组胺呈双相升高。第一个峰值出现在0.5h，也就是更过敏期，第二个峰值出现在24h。组胺和血清素是引起血浆渗出物过敏性增加的唯一原因。前列腺素和白三烯没有任何明显的变化。第二阶段组胺对眼袋内白细胞积累有抑制作用。在这方面，已经证明环氧化酶抑制剂对白细胞积累的众所周知的抑制作用是通过抑制炎症组织中E_2中前列腺的产生来增加组胺的产生。(4)酶莫桑诱导的炎症：在即刻期，白三烯虽然在炎症位点产生活性，但没有明确的作用，而组胺和5 -羟色胺主要负责血浆渗出。(5)羧甲基纤维素溶液：该溶液具有合适的粘度和对组织的低刺激性，被证明是一种有用的溶剂，可以容纳各种炎症剂。少

1. AIM OF THE PROJECT: The attempt was made to investigate roles of various putative chemical mediators of inflammation and allergy with the aid of various air pouch models of inflammation developed in this laboratory.2. RESULTS AND CNCLUSION: (1)Carrageenin-induced inflammation: In the inflmmatory site bradykinin levels were closely correlated with the intensity of plasma exudation in the early stage up to 1.5hr. Treatment with a kallikrein inhibitor decreased bradykinin levels in parallel with the decrease in the exuldation. These results clearly indicate the definite role of bradykinin in the early stage of carrageenin-induced inflammation. (2)Kaolin-induced inflammation: It was confirmed that daolin suspension infused into the air pouch caused plasma exudation through generation of bradykinin via activation of kallikrein-kinin system. (3)allergic inflammation: Histamine showed biphasic increase in the pouch fluid in the allergic model of inflammation. The first maximum, i.e. the an … More aphylactic phase, was at 0.5h and the second peak was at 24hr. histamine and serotonin were exclusively responsible for the anaphylactic increase of plasma exudation. Prostaglandins and leukotrienes did not have any significant fole at all. Histamine in the second phase exerted inhibitory effect on leukocyte accumulation in the pouch. In this connection, it was demonstrated that well-known inhibitory effect of cyclooxygenase inhibitors on leukocyte accumulation was derived by increased production of histamine via suppression of the production of prostagland in E_2 in the inflammatory tissues. (4)Zymosan-induced inflammation: In the immediate phase leukotrienes have no definite role in spite of the active generation in the imflammatory locus, but histamine and serotonin are mainly responsible for the plasma exudation. (5)Carboxymethylcellulose solution: The solution was proved to be useful as a solvent to hold various inflammatory agents because of its suitable viscosity and low irritability to the tissues. Less