Vitamin D induces a dynamic and celltype-specific modulation of the 3D- chromatin structure

维生素 D 诱导 3D 染色质结构的动态和细胞类型特异性调节

• 批准号: 437173059
• 负责人: Dr. Sabine Seuter
• 金额: --
• 依托单位: Vascular Research Center
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/437173059?language=en
• 关键词: Vitamin; induces; dynamic; celltype; specific

This project aims to prove that the insulation of DNA loci by CTCF is the basis for a context-dependent vitamin D signaling. Modern, state- of-the art methods like HiChIP, ChIPmentation, RNA-seq, RIME and CRISPR/Cas9 will be used for this approach. The main objective is to study the extent to which the situation- and tissue-specific gene regulation by vitamin D is determined or modulated by the insulator function of CTCF.

该项目旨在证明CTCF对DNA位点的绝缘是上下文依赖的维生素D信号传导的基础。现代最先进的方法如HiChIP、ChIPmentation、RNA-seq、RIME和CRISPR/Cas9将用于该方法。主要目的是研究在何种程度上的情况和组织特异性的基因调控维生素D是由CTCF的绝缘子功能确定或调制。